Literature DB >> 8789807

An RT-PCR analysis of mRNA for growth factor receptors in damaged and control sensory epithelia of rat utricles.

L D Saffer1, R Gu, J T Corwin.   

Abstract

Sensory epithelia from normal rat utricles and those cultured with and without neomycin treatment were assayed for the presence of growth factor receptor mRNAs by RT-PCR (reverse transcriptase-polymerase chain reaction). Both undamaged and damaged utricles showed mRNA for Insulin receptor, IGF-I receptor, FGF receptor 1, EGF receptor, and PDGF alpha receptor. Neomycin-damaged sensory epithelia showed less PDGF alpha receptor mRNA than undamaged epithelia, suggesting that this message by expressed at higher copy levels in hair cells than in supporting cells. Consistent with that hypothesis, immunohistochemistry revealed much stronger PDGF alpha receptor staining in the hair cells than in the supporting cells. Preliminary evidence suggests that IGF-I receptor message also may be lowered in neomycin-damaged epithelia.

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Year:  1996        PMID： 8789807     DOI： 10.1016/0378-5955(95)00228-6

Source DB:  PubMed          Journal:  Hear Res        ISSN： 0378-5955            Impact factor:   3.208


  17 in total

1.  Differentiation of mammalian vestibular hair cells from conditionally immortal, postnatal supporting cells.

Authors:  P Lawlor; W Marcotti; M N Rivolta; C J Kros; M C Holley
Journal:  J Neurosci       Date:  1999-11-01       Impact factor: 6.167

2.  Intracellular signals that control cell proliferation in mammalian balance epithelia: key roles for phosphatidylinositol-3 kinase, mammalian target of rapamycin, and S6 kinases in preference to calcium, protein kinase C, and mitogen-activated protein kinase.

Authors:  M Montcouquiol; J T Corwin
Journal:  J Neurosci       Date:  2001-01-15       Impact factor: 6.167

3.  M-like K+ currents in type I hair cells and calyx afferent endings of the developing rat utricle.

Authors:  Karen M Hurley; Sophie Gaboyard; Meng Zhong; Steven D Price; Julian R A Wooltorton; Anna Lysakowski; Ruth Anne Eatock
Journal:  J Neurosci       Date:  2006-10-04       Impact factor: 6.167

Review 4.  [Regenerative medicine in the treatment of sensorineural hearing loss].

Authors:  H Löwenheim; J Waldhaus; B Hirt; S Sandke; M Müller
Journal:  HNO       Date:  2008-03       Impact factor: 1.284

5.  Expression of growth factors and their receptors in the postnatal rat cochlea.

Authors:  B Malgrange; B Rogister; P P Lefebvre; C Mazy-Servais; A A Welcher; C Bonnet; R Y Hsu; J M Rigo; T R Van De Water; G Moonen
Journal:  Neurochem Res       Date:  1998-08       Impact factor: 3.996

6.  Brief treatments with forskolin enhance s-phase entry in balance epithelia from the ears of rats.

Authors:  M Montcouquiol; J T Corwin
Journal:  J Neurosci       Date:  2001-02-01       Impact factor: 6.167

Review 7.  Inner ear supporting cells: rethinking the silent majority.

Authors:  Guoqiang Wan; Gabriel Corfas; Jennifer S Stone
Journal:  Semin Cell Dev Biol       Date:  2013-03-29       Impact factor: 7.727

8.  Primary culture and plasmid electroporation of the murine organ of Corti.

Authors:  Mark Parker; Aurore Brugeaud; Albert S B Edge
Journal:  J Vis Exp       Date:  2010-02-04       Impact factor: 1.355

9.  "In-bone" utricle cultures--a simplified, atraumatic technique for in situ cultures of the adult mouse (Mus musculus) utricle.

Authors:  Henry C Ou; Vincent Lin; Edwin W Rubel
Journal:  Otol Neurotol       Date:  2013-02       Impact factor: 2.311

Review 10.  Regeneration of hair cells in the mammalian vestibular system.

Authors:  Wenyan Li; Dan You; Yan Chen; Renjie Chai; Huawei Li
Journal:  Front Med       Date:  2016-05-17       Impact factor: 4.592
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