Literature DB >> 8789047

New PCR assay for rapid and quantitative detection of human cytomegalovirus in cerebrospinal fluid.

J U Vogel1, J Cinatl, A Lux, B Weber, A J Driesel, H W Doerr.   

Abstract

Rapid Chelex extraction combined with an automated hybridization assay for the detection of PCR-amplified human cytomegalovirus DNA from cerebrospinal fluid was established. Quantitation of DNA was performed with a plasmid being used as an external standard. The detection limit was 10 copies per microliter. Quantitative detection of human cytomegalovirus DNA could be achieved over a range from 10 to 10(4) copies per microliter.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8789047      PMCID： PMC228829          DOI： 10.1128/jcm.34.2.482-483.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.

Authors:  P S Walsh; D A Metzger; R Higuchi
Journal:  Biotechniques       Date:  1991-04       Impact factor: 1.993

2.  Comparison of two hybridization assays for the rapid detection of PCR amplified HSV genome sequences from cerebrospinal fluid.

Authors:  A Sakrauski; B Weber; H H Kessler; K Pierer; H W Doerr
Journal:  J Virol Methods       Date:  1994-12       Impact factor: 2.014

3.  The AIDS dementia complex: II. Neuropathology.

Authors:  B A Navia; E S Cho; C K Petito; R W Price
Journal:  Ann Neurol       Date:  1986-06       Impact factor: 10.422

4.  Polymerase chain reaction to detect cytomegalovirus DNA in the cerebrospinal fluid of neonates with congenital infection.

Authors:  J Troendle Atkins; G J Demmler; W D Williamson; J M McDonald; A S Istas; G J Buffone
Journal:  J Infect Dis       Date:  1994-06       Impact factor: 5.226

5.  Rapid detection of cytomegalovirus DNA in cerebrospinal fluid of AIDS patients with neurologic disorders.

Authors:  J Gozlan; J M Salord; E Roullet; M Baudrimont; F Caburet; O Picard; M C Meyohas; C Duvivier; C Jacomet; J C Petit
Journal:  J Infect Dis       Date:  1992-12       Impact factor: 5.226

6.  Low correlation of human cytomegalovirus DNA amplification by polymerase chain reaction with cytomegalovirus disease in organ transplant recipients.

Authors:  B Weber; U Nestler; W Ernst; H Rabenau; J Braner; A Birkenbach; E H Scheuermann; W Schoeppe; H W Doerr
Journal:  J Med Virol       Date:  1994-06       Impact factor: 2.327

7.  Detection of cytomegalovirus in bronchial lavage and urine using a monoclonal antibody to an HCMV early nuclear protein.

Authors:  C Schacherer; W Braun; G Bauer; H W Doerr
Journal:  Infection       Date:  1988 Sep-Oct       Impact factor: 3.553

8.  Isolation of HTLV-III from cerebrospinal fluid and neural tissues of patients with neurologic syndromes related to the acquired immunodeficiency syndrome.

Authors:  D D Ho; T R Rota; R T Schooley; J C Kaplan; J D Allan; J E Groopman; L Resnick; D Felsenstein; C A Andrews; M S Hirsch
Journal:  N Engl J Med       Date:  1985-12-12       Impact factor: 91.245
  8 in total
  2 in total

1.  Unusual course of herpes simplex virus encephalitis after acyclovir therapy.

Authors:  W Preiser; B Weber; G Klös; P A Fischer; H W Doerr
Journal:  Infection       Date:  1996 Sep-Oct       Impact factor: 3.553

Review 2.  Quantitation of cytomegalovirus: methodologic aspects and clinical applications.

Authors:  M Boeckh; G Boivin
Journal:  Clin Microbiol Rev       Date:  1998-07       Impact factor: 26.132
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.