Literature DB >> 8788275

Immortalisation of human urothelial cells.

J L Petzoldt1, I M Leigh, P G Duffy, C Sexton, J R Masters.   

Abstract

A cell line derived from the urothelium lining the ureter of a 12-year-old girl was immortalised using a temperature-sensitive SV40 large T-antigen gene construct, and designated UROtsa. Following immortalisation, UROtsa cells expressed SV40 large T-antigen, but did not acquire characteristics of neoplastic transformation, including growth in soft agar or the development of tumours in nude mice. Metaphase spreads had a normal chromosomal appearance and number. UROtsa cells remained permissive for cell growth at 39 degrees C, indicating that they did not retain temperature sensitivity. UROtsa provides an in vitro model of "normal" urothelium.

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Year:  1995        PMID： 8788275     DOI： 10.1007/bf00698738

Source DB:  PubMed          Journal:  Urol Res        ISSN： 0300-5623


  11 in total

1.  THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.

Authors:  L HAYFLICK
Journal:  Exp Cell Res       Date:  1965-03       Impact factor: 3.905

2.  Characterization of human uroepithelial cells immortalized in vitro by simian virus 40.

Authors:  B J Christian; L J Loretz; T D Oberley; C A Reznikoff
Journal:  Cancer Res       Date:  1987-11-15       Impact factor: 12.701

3.  Cell lines established by a temperature-sensitive simian virus 40 large-T-antigen gene are growth restricted at the nonpermissive temperature.

Authors:  P S Jat; P A Sharp
Journal:  Mol Cell Biol       Date:  1989-04       Impact factor: 4.272

4.  Neoplastic transformation of SV40-immortalized human urinary tract epithelial cells by in vitro exposure to 3-methylcholanthrene.

Authors:  C A Reznikoff; L J Loretz; B J Christian; S Q Wu; L F Meisner
Journal:  Carcinogenesis       Date:  1988-08       Impact factor: 4.944

5.  Carcinogen-induced amplification of SV40 DNA inserted at 9q12-21.1 associated with chromosome breakage, deletions, and translocations in human uroepithelial cell transformation in vitro.

Authors:  C Kao; S Q Wu; S DeVries; W S Reznikoff; F M Waldman; C A Reznikoff
Journal:  Genes Chromosomes Cancer       Date:  1993-11       Impact factor: 5.006

6.  In vitro radiation-induced neoplastic progression of low-grade uroepithelial tumors.

Authors:  S Pazzaglia; X R Chen; C B Aamodt; S Q Wu; C Kao; K W Gilchrist; R Oyasu; C A Reznikoff; M A Ritter
Journal:  Radiat Res       Date:  1994-04       Impact factor: 2.841

Review 7.  A molecular genetic model of human bladder carcinogenesis.

Authors:  C A Reznikoff; C Kao; E M Messing; M Newton; S Swaminathan
Journal:  Semin Cancer Biol       Date:  1993-06       Impact factor: 15.707

8.  Chromosome losses in tumorigenic revertants of EJ/ras-expressing somatic cell hybrids.

Authors:  C I Pratt; S Q Wu; M Bhattacharya; C Kao; K W Gilchrist; C A Reznikoff
Journal:  Cancer Genet Cytogenet       Date:  1992-04

9.  Tumorigenic transformation and neoplastic progression of human uroepithelial cells after exposure in vitro to 4-aminobiphenyl or its metabolites.

Authors:  E A Bookland; S Swaminathan; R Oyasu; K W Gilchrist; M Lindstrom; C A Reznikoff
Journal:  Cancer Res       Date:  1992-03-15       Impact factor: 12.701

10.  Culture and characterisation of human urothelium in vivo and in vitro.

Authors:  J L Petzoldt; I M Leigh; P G Duffy; J R Masters
Journal:  Urol Res       Date:  1994
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  41 in total

1.  Human urothelial cell lines as potential models for studying cannabinoid and excitatory receptor interactions in the urinary bladder.

Authors:  Evangelia Bakali; Ruth A Elliott; Anthony H Taylor; David G Lambert; Jonathon M Willets; Douglas G Tincello
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  2014-03-21       Impact factor: 3.000

2.  Beclin-1 expression in normal bladder and in Cd2+ and As3+ exposed and transformed human urothelial cells (UROtsa).

Authors:  Jennifer L Larson; Seema Somji; Xu Dong Zhou; Mary Ann Sens; Scott H Garrett; Donald A Sens; Jane R Dunlevy
Journal:  Toxicol Lett       Date:  2010-03-03       Impact factor: 4.372

3.  A time-series analysis of altered histone H3 acetylation and gene expression during the course of MMAIII-induced malignant transformation of urinary bladder cells.

Authors:  Jinqiu Zhu; Jie Wang; Xushen Chen; Maria Tsompana; Daniel Gaile; Michael Buck; Xuefeng Ren
Journal:  Carcinogenesis       Date:  2017-04-01       Impact factor: 4.944

4.  Monomethylarsonous acid induces transformation of human bladder cells.

Authors:  Tiffany G Bredfeldt; Bhumasamudram Jagadish; Kylee E Eblin; Eugene A Mash; A Jay Gandolfi
Journal:  Toxicol Appl Pharmacol       Date:  2006-06-27       Impact factor: 4.219

5.  Bacterial lysis liberates the neutrophil migration suppressor YbcL from the periplasm of uropathogenic Escherichia coli.

Authors:  Megan E Lau; Elizabeth S Danka; Kristin M Tiemann; David A Hunstad
Journal:  Infect Immun       Date:  2014-09-02       Impact factor: 3.441

6.  Nrf2 protects human bladder urothelial cells from arsenite and monomethylarsonous acid toxicity.

Authors:  Xiao-Jun Wang; Zheng Sun; Weimin Chen; Kylee E Eblin; Jay A Gandolfi; Donna D Zhang
Journal:  Toxicol Appl Pharmacol       Date:  2007-08-07       Impact factor: 4.219

7.  Frequency of activating mutations in FGFR2 exon 7 in bladder tumors from patients with early-onset and regular-onset disease.

Authors:  Christine Spiegelberg; Johannes Giedl; Nadine T Gaisa; Anja Rogler; Marc-Oliver Riener; Thomas Filbeck; Maximilian Burger; Petra Ruemmele; Arndt Hartmann; Robert Stoehr
Journal:  Int J Clin Exp Pathol       Date:  2014-03-15

8.  Transcriptional Modulation of the ERK1/2 MAPK and NF-κB Pathways in Human Urothelial Cells After Trivalent Arsenical Exposure: Implications for Urinary Bladder Cancer.

Authors:  Kathryn A Bailey; Kathleen Wallace; Lisa Smeester; Sheau-Fung Thai; Douglas C Wolf; Stephen W Edwards; Rebecca C Fry
Journal:  J Can Res Updates       Date:  2012-08-21

9.  Requirement of arsenic biomethylation for oxidative DNA damage.

Authors:  Chikara Kojima; Dario C Ramirez; Erik J Tokar; Seiichiro Himeno; Zuzana Drobná; Miroslav Stýblo; Ronald P Mason; Michael P Waalkes
Journal:  J Natl Cancer Inst       Date:  2009-12-16       Impact factor: 13.506

10.  Frankincense oil derived from Boswellia carteri induces tumor cell specific cytotoxicity.

Authors:  Mark Barton Frank; Qing Yang; Jeanette Osban; Joseph T Azzarello; Marcia R Saban; Ricardo Saban; Richard A Ashley; Jan C Welter; Kar-Ming Fung; Hsueh-Kung Lin
Journal:  BMC Complement Altern Med       Date:  2009-03-18       Impact factor: 3.659
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