Literature DB >> 8787922

Simple in vitro translation assay to analyze inhibitors of rhinovirus proteases.

B A Heinz1, J Tang, J M Labus, F W Chadwell, S W Kaldor, M Hammond.   

Abstract

We have developed a simple in vitro translation method to analyze compounds that inhibit the rhinovirus 3C protease in peptide substrate assays but demonstrate no antiviral activity. This complementary assay, which provides both qualitative and quantitative results, detects the inhibition of the 3CD protease in the native polyprotein form.

Mesh：

Substances：

Year:  1996        PMID： 8787922      PMCID： PMC163099     

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  19 in total

1.  Translation of poliovirus RNA in vitro: changes in cleavage pattern and initiation sites by ribosomal salt wash.

Authors:  B A Brown; E Ehrenfeld
Journal:  Virology       Date:  1979-09       Impact factor: 3.616

2.  In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase.

Authors:  M F Ypma-Wong; B L Semler
Journal:  Nucleic Acids Res       Date:  1987-03-11       Impact factor: 16.971

3.  Protein 3CD is the major poliovirus proteinase responsible for cleavage of the P1 capsid precursor.

Authors:  M F Ypma-Wong; P G Dewalt; V H Johnson; J G Lamb; B L Semler
Journal:  Virology       Date:  1988-09       Impact factor: 3.616

4.  Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.

Authors:  S van der Werf; J Bradley; E Wimmer; F W Studier; J J Dunn
Journal:  Proc Natl Acad Sci U S A       Date:  1986-04       Impact factor: 11.205

Review 5.  Fine-needle aspiration biopsy of the breast.

Authors:  T S Kline
Journal:  Am Fam Physician       Date:  1995-11-15       Impact factor: 3.292

6.  Proteolytic events in replication of animal viruses.

Authors:  B D Korant; N L Chow; M O Lively; J C Powers
Journal:  Ann N Y Acad Sci       Date:  1980       Impact factor: 5.691

7.  Poliovirus proteinase 3C: large-scale expression, purification, and specific cleavage activity on natural and synthetic substrates in vitro.

Authors:  M J Nicklin; K S Harris; P V Pallai; E Wimmer
Journal:  J Virol       Date:  1988-12       Impact factor: 5.103

8.  The antiviral compound enviroxime targets the 3A coding region of rhinovirus and poliovirus.

Authors:  B A Heinz; L M Vance
Journal:  J Virol       Date:  1995-07       Impact factor: 5.103

9.  Purification of recombinant human rhinovirus 14 3C protease expressed in Escherichia coli.

Authors:  G M Birch; T Black; S K Malcolm; M T Lai; R E Zimmerman; S R Jaskunas
Journal:  Protein Expr Purif       Date:  1995-10       Impact factor: 1.650

Review 10.  Picornavirus inhibitors.

Authors:  L Carrasco
Journal:  Pharmacol Ther       Date:  1994       Impact factor: 12.310
View more
  5 in total

Review 1.  Protease inhibitors as antiviral agents.

Authors:  A K Patick; K E Potts
Journal:  Clin Microbiol Rev       Date:  1998-10       Impact factor: 26.132

2.  Dual inhibition of human rhinovirus 2A and 3C proteases by homophthalimides.

Authors:  Q M Wang; R B Johnson; L N Jungheim; J D Cohen; E C Villarreal
Journal:  Antimicrob Agents Chemother       Date:  1998-04       Impact factor: 5.191

3.  The refined crystal structure of the 3C gene product from hepatitis A virus: specific proteinase activity and RNA recognition.

Authors:  E M Bergmann; S C Mosimann; M M Chernaia; B A Malcolm; M N James
Journal:  J Virol       Date:  1997-03       Impact factor: 5.103

4.  Inhibition of human rhinovirus-induced cytokine production by AG7088, a human rhinovirus 3C protease inhibitor.

Authors:  L S Zalman; M A Brothers; P S Dragovich; R Zhou; T J Prins; S T Worland; A K Patick
Journal:  Antimicrob Agents Chemother       Date:  2000-05       Impact factor: 5.191

5.  In vitro antiviral activity of AG7088, a potent inhibitor of human rhinovirus 3C protease.

Authors:  A K Patick; S L Binford; M A Brothers; R L Jackson; C E Ford; M D Diem; F Maldonado; P S Dragovich; R Zhou; T J Prins; S A Fuhrman; J W Meador; L S Zalman; D A Matthews; S T Worland
Journal:  Antimicrob Agents Chemother       Date:  1999-10       Impact factor: 5.191
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.