Literature DB >> 8787410

Rapid purification of the oxygenase component of toluene dioxygenase from a polyol-responsive monoclonal antibody.

N A Lynch1, H Jiang, D T Gibson.   

Abstract

A monoclonal antibody designated 302 beta that is specific for the beta subunit of the oxygenase component (ISPTOL) of toluene dioxygenase from Pseudomonas putida F1 was used to prepare an immunoaffinity column. ISPTOL in cell extracts of Escherichia coli JM109(pDTG611) bound to the column, and an enzyme-linked immunosorbent elution-screening assay with different combinations of polyols and kosmotropic anions was used to determine the conditions necessary for recovery of active enzyme. Elution from an 8-ml antibody column with 50 mM 2-(N-morpholino)ethanesulfonate buffer (pH 6.8) containing 50% ethylene glycol, 1.0 M ammonium sulfate, 1.0 mM dithiothreitol, and 0.2 mM ferrous ammonium sulfate gave approximately 2 mg of ISPTOL with a specific activity that was more than 300 times the specific activity previously obtained.

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Year:  1996        PMID： 8787410      PMCID： PMC167991          DOI： 10.1128/aem.62.6.2133-2137.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

Review 1.  Aromatic hydrocarbon degradation: a molecular approach.

Authors:  G J Zylstra; D T Gibson
Journal:  Genet Eng (N Y)       Date:  1991

2.  Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase.

Authors:  N E Thompson; D A Hager; R R Burgess
Journal:  Biochemistry       Date:  1992-08-04       Impact factor: 3.162

3.  Toluene dioxygenase from Pseudomonas putida F1.

Authors:  L P Wackett
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

4.  Formation of (+)-cis-2,3-dihydroxy-1-methylcyclohexa-4,6-diene from toluene by Pseudomonas putida.

Authors:  D T Gibson; M Hensley; H Yoshioka; T J Mabry
Journal:  Biochemistry       Date:  1970-03-31       Impact factor: 3.162

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in Pseudomonas putida F1.

Authors:  J C Spain; G J Zylstra; C K Blake; D T Gibson
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

7.  Purification and characterization of the oxygenase component of biphenyl 2,3-dioxygenase from Pseudomonas sp. strain LB400.

Authors:  J D Haddock; D T Gibson
Journal:  J Bacteriol       Date:  1995-10       Impact factor: 3.490

8.  Recombinant Escherichia coli strains synthesize active forms of naphthalene dioxygenase and its individual alpha and beta subunits.

Authors:  W C Suen; D T Gibson
Journal:  Gene       Date:  1994-05-27       Impact factor: 3.688

9.  Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.

Authors:  G J Zylstra; L P Wackett; D T Gibson
Journal:  Appl Environ Microbiol       Date:  1989-12       Impact factor: 4.792

10.  Oxidation of nitrotoluenes by toluene dioxygenase: evidence for a monooxygenase reaction.

Authors:  J B Robertson; J C Spain; J D Haddock; D T Gibson
Journal:  Appl Environ Microbiol       Date:  1992-08       Impact factor: 4.792
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  10 in total

1.  Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

Authors:  R E Parales; K Lee; S M Resnick; H Jiang; D J Lessner; D T Gibson
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

2.  Production and characterization of monoclonal antibodies to estrogen-related receptor alpha (ERRα) and use in immunoaffinity chromatography.

Authors:  Amanda M Esch; Nancy E Thompson; Jennifer A Lamberski; Janet E Mertz; Richard R Burgess
Journal:  Protein Expr Purif       Date:  2012-05-04       Impact factor: 1.650

Review 3.  Epoxy Coenzyme A Thioester pathways for degradation of aromatic compounds.

Authors:  Wael Ismail; Johannes Gescher
Journal:  Appl Environ Microbiol       Date:  2012-05-11       Impact factor: 4.792

4.  The alpha subunit of toluene dioxygenase from Pseudomonas putida F1 can accept electrons from reduced FerredoxinTOL but is catalytically inactive in the absence of the beta subunit.

Authors:  H Jiang; R E Parales; D T Gibson
Journal:  Appl Environ Microbiol       Date:  1999-01       Impact factor: 4.792

5.  Substrate specificities of hybrid naphthalene and 2,4-dinitrotoluene dioxygenase enzyme systems.

Authors:  R E Parales; M D Emig; N A Lynch; D T Gibson
Journal:  J Bacteriol       Date:  1998-05       Impact factor: 3.490

6.  Purification, characterization, and crystallization of the components of a biphenyl dioxygenase system from Sphingobium yanoikuyae B1.

Authors:  C L Yu; W Liu; D J Ferraro; E N Brown; J V Parales; S Ramaswamy; G J Zylstra; D T Gibson; R E Parales
Journal:  J Ind Microbiol Biotechnol       Date:  2007-01-09       Impact factor: 3.346

7.  Latex clearing protein-an oxygenase cleaving poly(cis-1,4-isoprene) rubber at the cis double bonds.

Authors:  Sebastian Hiessl; Dietrich Böse; Sylvia Oetermann; Jessica Eggers; Jörg Pietruszka; Alexander Steinbüchel
Journal:  Appl Environ Microbiol       Date:  2014-06-13       Impact factor: 4.792

8.  Open reading frame 3, which is adjacent to the mycocerosic acid synthase gene, is expressed as an acyl coenzyme A synthase in Mycobacterium bovis BCG.

Authors:  A M Fitzmaurice; P E Kolattukudy
Journal:  J Bacteriol       Date:  1997-04       Impact factor: 3.490

9.  Aspartate 205 in the catalytic domain of naphthalene dioxygenase is essential for activity.

Authors:  R E Parales; J V Parales; D T Gibson
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

10.  Antigen-binding properties of monoclonal antibodies reactive with EBNA1 and use in immunoaffinity chromatography.

Authors:  Sarah J Duellman; Richard R Burgess
Journal:  PLoS One       Date:  2009-02-26       Impact factor: 3.240
  10 in total

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