Literature DB >> 8785488

Large scale preparation of PA-oligosaccharides from glycoproteins using an improved extraction method.

K Tokugawa1, S Oguri, M Takeuchi.   

Abstract

We have developed a new method for the large scale preparation of pyridylaminated (PA-) oligosaccharides from glycoproteins. Phenol/chloroform extraction was adapted for the removal of protein and excess 2-aminopyridine, improving the efficiency of preparation. From a 2.5 g sample of human apo-transferrin, 25-30 mu mol of agalacto biantennary PA-oligosaccharide could be obtained. By increasing the concentration of PA-oligosaccharide substrate, we were able to detect a very low level of N-acetylglucosaminlytransferase IV activity in CHO cell extracts.

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Year:  1996        PMID： 8785488

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  4 in total

1.  High-performance liquid chromatography of pyridylaminated saccharides.

Authors:  S Hase
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

2.  Determination of N-acetylglucosaminyltransferases III, IV and V in normal and hepatoma tissues of rats.

Authors:  A Nishikawa; J Gu; S Fujii; N Taniguchi
Journal:  Biochim Biophys Acta       Date:  1990-09-14

3.  Reexamination of the pyridylamination used for fluorescence labeling of oligosaccharides and its application to glycoproteins.

Authors:  S Hase; T Ibuki; T Ikenaka
Journal:  J Biochem       Date:  1984-01       Impact factor: 3.387

4.  Structure determination by 1H NMR spectroscopy of (sulfated) sialylated N-linked carbohydrate chains released from porcine thyroglobulin by peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase-F.

Authors:  P de Waard; A Koorevaar; J P Kamerling; J F Vliegenthart
Journal:  J Biol Chem       Date:  1991-03-05       Impact factor: 5.157
  4 in total
  10 in total

1.  Bisecting GlcNAc structure is implicated in suppression of stroma-dependent haemopoiesis in transgenic mice expressing N-acetylglucosaminyltransferase III.

Authors:  M Yoshimura; Y Ihara; T Nishiura; Y Okajima; M Ogawa; H Yoshida; M Suzuki; K Yamamura; Y Kanakura; Y Matsuzawa; N Taniguchi
Journal:  Biochem J       Date:  1998-05-01       Impact factor: 3.857

2.  N-Glycosylation profiling of recombinant mouse extracellular superoxide dismutase produced in Chinese hamster ovary cells.

Authors:  Hiroaki Korekane; Atsuko Korekane; Yoshiki Yamaguchi; Masaki Kato; Yasuhide Miyamoto; Akio Matsumoto; Tomoko Hasegawa; Keiichiro Suzuki; Naoyuki Taniguchi; Tomomi Ookawara
Journal:  Glycoconj J       Date:  2011-05-15       Impact factor: 2.916

3.  Ectopic expression of N-acetylglucosaminyltransferase III in transgenic hepatocytes disrupts apolipoprotein B secretion and induces aberrant cellular morphology with lipid storage.

Authors:  Y Ihara; M Yoshimura; E Miyoshi; A Nishikawa; A S Sultan; S Toyosawa; A Ohnishi; M Suzuki; K Yamamura; N Ijuhin; N Taniguchi
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-03       Impact factor: 11.205

4.  Structural and quantitative evidence for dynamic glycome shift on production of induced pluripotent stem cells.

Authors:  Kayo Hasehira; Hiroaki Tateno; Yasuko Onuma; Yuzuru Ito; Makoto Asashima; Jun Hirabayashi
Journal:  Mol Cell Proteomics       Date:  2012-09-27       Impact factor: 5.911

5.  Kinetic properties and substrate specificities of two recombinant human N-acetylglucosaminyltransferase-IV isozymes.

Authors:  Suguru Oguri; Aruto Yoshida; Mari T Minowa; Makoto Takeuchi
Journal:  Glycoconj J       Date:  2006-11       Impact factor: 2.916

6.  Electrospray mass spectrometry and fragmentation of N-linked carbohydrates derivatized at the reducing terminus.

Authors:  D J Harvey
Journal:  J Am Soc Mass Spectrom       Date:  2000-10       Impact factor: 3.109

7.  Physiological and glycomic characterization of N-acetylglucosaminyltransferase-IVa and -IVb double deficient mice.

Authors:  Shinji Takamatsu; Aristotelis Antonopoulos; Kazuaki Ohtsubo; David Ditto; Yasunori Chiba; Dzung T Le; Howard R Morris; Stuart M Haslam; Anne Dell; Jamey D Marth; Naoyuki Taniguchi
Journal:  Glycobiology       Date:  2009-12-16       Impact factor: 4.313

8.  Abnormal biantennary sugar chains are expressed in human chorionic gonadotropin produced in the choriocarcinoma cell line, JEG-3.

Authors:  Shinji Takamatsu; Toshiyuki Katsumata; Noboru Inoue; Toshinori Watanabe; Yasuhisa Fujibayashi; Makoto Takeuchi
Journal:  Glycoconj J       Date:  2004       Impact factor: 2.916

9.  Mass isotopomer analysis of metabolically labeled nucleotide sugars and N- and O-glycans for tracing nucleotide sugar metabolisms.

Authors:  Kazuki Nakajima; Emi Ito; Kazuaki Ohtsubo; Ken Shirato; Rina Takamiya; Shinobu Kitazume; Takashi Angata; Naoyuki Taniguchi
Journal:  Mol Cell Proteomics       Date:  2013-05-29       Impact factor: 5.911

10.  Structure determination of a sulfated N-glycans, candidate for a precursor of the selectin ligand in bovine lung.

Authors:  Tomonori Murakami; Shunji Natsuka; Shin-Ichi Nakakita; Sumihiro Hase
Journal:  Glycoconj J       Date:  2007-03-14       Impact factor: 3.009
  10 in total

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