Literature DB >> 8785481

Identification of a GDP-Fuc:Gal beta 1-3GalNAc-R (Fuc to Gal) alpha 1-2 fucosyltransferase and a GDP-Fuc:Gal beta 1-4GlcNAc (Fuc to GlcNAc) alpha 1-3 fucosyltransferase in connective tissue of the snail Lymnaea stagnalis.

H Mulder1, H Schachter, J R Thomas, K M Halkes, J P Kamerling, J F Vliegenthart.   

Abstract

Connective tissue of the freshwater pulmonate Lymnaea stagnalis was shown to contain fucosyltransferase activity capable of transferring fucose from GDP-Fuc in alpha 1 -2 linkage to terminal Gal of type 3 (Gal beta 1-3GalNAc) acceptors, and in alpha 1-3 linkage to GlcNAc ot type 2 (Gal beta 1-4GlcNAc) acceptors. The alpha 1-2 fucosyltransferase was active with Gal beta 1-3GalNAc beta 1-OCH2CH=CH2 (Km = 12mM, V(max) = 1.3 mUml-1) and Gal beta 1-3GalNAc (km =20 mM, V(max) = 2.1 mUml-1), whereas the alpha 1-3 fucosyltransferase was active with Gal beta 1-4GlcNAc (Km = 23 mM, V(max) = 1.1 mUml-1). The products formed from from Gal beta 1-3GalNAc beta 1-OCH2CH=CH2 and Gal beta 1-4GlcNAc were purified by high performance liquid chromatography, and identified by 500 MHz 1H-NMR spectroscopy and methylation analysis to be Fucalpha1-2Gal beta 1-3GalNAc beta 1-OCH2CH=CH2 and Gal beta 1-4(Fucalpha1-3)GlcNAc, respectively. Competition experiments suggest that the two fucosyltransferase activities are due to two distinct enzymes.

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Year:  1996        PMID： 8785481     DOI： 10.1007/bf01049686

Source DB:  PubMed          Journal:  Glycoconj J        ISSN： 0282-0080            Impact factor:   2.916


  22 in total

1.  Molecular cloning, sequence, and expression of a human GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase cDNA that can form the H blood group antigen.

Authors:  R D Larsen; L K Ernst; R P Nair; J B Lowe
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

2.  500-MHz 1H-n.m.r. and conformational studies of fucosyloligosaccharides recognised by monoclonal antibodies with specificities related to Le(a), Le(b), and SSEA-1.

Authors:  E F Hounsell; N J Jones; H C Gooi; T Feizi; A S Donald; J Feeney
Journal:  Carbohydr Res       Date:  1988-07-15       Impact factor: 2.104

3.  Stable expression of blood group H determinants and GDP-L-fucose: beta-D-galactoside 2-alpha-L-fucosyltransferase in mouse cells after transfection with human DNA.

Authors:  L K Ernst; V P Rajan; R D Larsen; M M Ruff; J B Lowe
Journal:  J Biol Chem       Date:  1989-02-25       Impact factor: 5.157

4.  Purification of H gene-encoded beta-galactoside alpha 1----2 fucosyltransferase from human serum.

Authors:  A Sarnesto; T Köhlin; J Thurin; M Blaszczyk-Thurin
Journal:  J Biol Chem       Date:  1990-09-05       Impact factor: 5.157

5.  Molecular cloning of a human genomic region containing the H blood group alpha(1,2)fucosyltransferase gene and two H locus-related DNA restriction fragments. Isolation of a candidate for the human Secretor blood group locus.

Authors:  S Rouquier; J B Lowe; R J Kelly; A L Fertitta; G G Lennon; D Giorgi
Journal:  J Biol Chem       Date:  1995-03-03       Impact factor: 5.157

6.  Solubilization and properties of GDP-fucose: xyloglucan 1,2-alpha-L-fucosyltransferase from pea epicotyl membranes.

Authors:  R Hanna; D A Brummell; A Camirand; A Hensel; E F Russell; G A Maclachlan
Journal:  Arch Biochem Biophys       Date:  1991-10       Impact factor: 4.013

7.  A cloned human cDNA determines expression of a mouse stage-specific embryonic antigen and the Lewis blood group alpha(1,3/1,4)fucosyltransferase.

Authors:  J F Kukowska-Latallo; R D Larsen; R P Nair; J B Lowe
Journal:  Genes Dev       Date:  1990-08       Impact factor: 11.361

8.  Primary structure determination of seven novel N-linked carbohydrate chains derived from hemocyanin of Lymnaea stagnalis. 3-O-methyl-D-galactose and N-acetyl-D-galactosamine as constituents of xylose-containing N-linked oligosaccharides in an animal glycoprotein.

Authors:  J A Van Kuik; R P Sijbesma; J P Kamerling; J F Vliegenthart; E J Wood
Journal:  Eur J Biochem       Date:  1987-12-01

9.  Conversion of GalNAc beta(1-4)GlcNAc beta-OMe into GalNAc beta (1-4)-[Fuc alpha(1-3)]GlcNAc beta-OMe using human milk alpha 3/4-fucosyltransferase. Synthesis of a novel terminal element in glycoprotein glycans.

Authors:  A A Bergwerff; J A van Kuik; W E Schiphorst; C A Koeleman; D H van den Eijnden; J P Kamerling; J F Vliegenthart
Journal:  FEBS Lett       Date:  1993-11-08       Impact factor: 4.124

10.  Isolation of a novel human alpha (1,3)fucosyltransferase gene and molecular comparison to the human Lewis blood group alpha (1,3/1,4)fucosyltransferase gene. Syntenic, homologous, nonallelic genes encoding enzymes with distinct acceptor substrate specificities.

Authors:  B W Weston; R P Nair; R D Larsen; J B Lowe
Journal:  J Biol Chem       Date:  1992-02-25       Impact factor: 5.157
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  5 in total

Review 1.  The joys of HexNAc. The synthesis and function of N- and O-glycan branches.

Authors:  H Schachter
Journal:  Glycoconj J       Date:  2000 Jul-Sep       Impact factor: 2.916

2.  The ruminant parasite Haemonchus contortus expresses an alpha1,3-fucosyltransferase capable of synthesizing the Lewis x and sialyl Lewis x antigens.

Authors:  R A DeBose-Boyd; A K Nyame; D P Jasmer; R D Cummings
Journal:  Glycoconj J       Date:  1998-08       Impact factor: 2.916

3.  Neutral N-glycan patterns of the gastropods Limax maximus, Cepaea hortensis, Planorbarius corneus, Arianta arbustorum and Achatina fulica.

Authors:  Martin Gutternigg; Sabine Bürgmayr; Gerald Pöltl; Judith Rudolf; Erika Staudacher
Journal:  Glycoconj J       Date:  2007-05-22       Impact factor: 2.916

4.  PROTEIN N-GLYCOSYLATION OF GASTROPODS.

Authors:  Erika Staudacher; Herwig Stepan; Martin Gutternigg
Journal:  Curr Top Biochem Res       Date:  2009-12

Review 5.  Mollusc N-glycosylation: Structures, Functions and Perspectives.

Authors:  Erika Staudacher
Journal:  Biomolecules       Date:  2021-12-03
  5 in total

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