Determination of some pharmacologically active phenolic acids in juices by high-performance liquid chromatography.

Several phenolic acids, e.g. caffeic acid, chlorogenic acid, ferulic acid, gallic acid and ellagic acid, which occur naturally, are inhibitors of carcinogenesis. In this paper we present a new method for the simultaneous determination of all of these compounds, except ellagic acid, in juices by reversed-phase high-performance liquid chromatography (HPLC) using ultraviolet detection and involving isocratic elution, and we have devised an HPLC method for the determination of ellagic acid in juices. The experimental results showed that cherry juice contains a high concentration of chlorogenic acid and the content of bound gallic acid in black and green grape juices is high compared to that of other phenolic acids.