| Literature DB >> 8782454 |
R H Begent1, M J Verhaar, K A Chester, J L Casey, A J Green, M P Napier, L D Hope-Stone, N Cushen, P A Keep, C J Johnson, R E Hawkins, A J Hilson, L Robson.
Abstract
We present a system for cancer targeting based on single-chain Fv (scFv) antibodies selected from combinatorial libraries, produced in bacteria and purified by using an engineered tag. Combinatorial libraries of scFv genes contain great diversity, and scFv antibodies with characteristics optimized for a particular task can be selected from them using filamentous bacteriophage. We illustrate the benefits of this system by imaging patients with carcinoembryonic antigen (CEA)-producing cancers using an iodine-123 labeled scFv anti-CEA selected for high affinity. All known tumor deposits were located, and advantages over current imaging technology are illustrated. ScFvs are produced in a cloned form and can be readily engineered to have localizing and therapeutic functions that will be applicable in cancer and other diseases.Entities:
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Year: 1996 PMID： 8782454 DOI： 10.1038/nm0996-979
Source DB: PubMed Journal: Nat Med ISSN： 1078-8956 Impact factor: 53.440