| Literature DB >> 8782348 |
S Bertagnoli1, J Gelfi, F Petit, J F Vautherot, D Rasschaert, S Laurent, G Le Gall, E Boilletot, J Chantal, C Boucraut-Baralon.
Abstract
In order to protect domestic and wild rabbits against RVHD, we constructed a recombinant vaccinia-RHDV virus, using the Copenhagen strain of the vaccinia virus. This recombinant virus expressed the RHDV capsid protein (VP60). Analysis of the expressed product showed that the recombinant protein, which is 60 kDa in size, was antigenic as revealed by its reactions in immunoprecipitation and indirect immunofluorescence with the antibodies raised against RHDV. The recombinant virus induced high level of RHDV specific antibodies in rabbits following immunization. Inoculations by both the intradermal and oral routes allow protection of animals against a challenge with virulent RHDV.Entities:
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Year: 1996 PMID： 8782348 DOI： 10.1016/0264-410x(95)00232-p
Source DB: PubMed Journal: Vaccine ISSN： 0264-410X Impact factor: 3.641