Evidence for separate serotonin and catecholamine compartments in human platelets.

Serotonin (5-HT) and the catecholamines (CA), noradrenaline (NA) and adrenaline (Ad), are platelet dense-granule constituents which influence platelet activity and vessel tone. Platelets accumulate 5-HT via an active process whilst CA uptake occurs mainly by passive diffusion. The platelet contents and collagen-stimulated efflux of 5-HT, NA and Ad were examined in normal individuals to establish whether relationships exist between these monoamines. Regression analysis revealed that platelet 5-HT was not related to platelet NA or Ad levels. Platelet NA, however, correlated positively with Ad (r = 0.61, P < 0.01). Collagen-induced release of all three monoamines occurred in a dose-dependent manner. The collagen EC50 values for 5-HT and CA release, however, differed and were greater for 5-HT release: 9.6 +/- 0.8 vs. 3.8 +/- 0.2 microgram/ml collagen, 5-HT vs. NA, P < 0.001; 9.6 +/- 0.8 vs. 3.9 +/- 0.5 microgram/ml, 5-HT vs Ad, P < 0.001. These data may reflect differences regarding the triggering mechanisms for 5-HT and CA release and provide evidence for separate compartments of intra-platelet 5-HT and CA and possibly distinct populations of 5-HT and CA containing dense granules and/or platelets.