Literature DB >> 8774915

A rapid method for detecting specific amplified PCR fragments in microtiter plates.

A Ortiz1, E Ritter.   

Abstract

A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or oligonucleotides by using a positively charged DEAE cellulose matrix. Amplification products are visualized directly in the matrix using immunoenzymatic methods or streptavidin-conjugated enzymes. The detection process can be carried out within 2 h, allows the processing of large sample sizes and can potentially be automated.

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Year:  1996        PMID： 8774915      PMCID： PMC146076          DOI： 10.1093/nar/24.16.3280

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  6 in total

1.  Direct analysis of polymerase chain reaction products using enzyme-linked immunosorbent assay techniques.

Authors:  A Landgraf; B Reckmann; A Pingoud
Journal:  Anal Biochem       Date:  1991-10       Impact factor: 3.365

2.  Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye.

Authors:  A Landgraf; B Reckmann; A Pingoud
Journal:  Anal Biochem       Date:  1991-03-02       Impact factor: 3.365

3.  Microplate hybridization of amplified viral DNA segment.

Authors:  S Inouye; R Hondo
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

4.  Quantification of polymerase chain reaction products by affinity-based hybrid collection.

Authors:  A C Syvänen; M Bengtström; J Tenhunen; H Söderlund
Journal:  Nucleic Acids Res       Date:  1988-12-09       Impact factor: 16.971

5.  A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens.

Authors:  G Nolasco; C de Blas; V Torres; F Ponz
Journal:  J Virol Methods       Date:  1993-12-15       Impact factor: 2.014

6.  Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.

Authors:  R K Saiki; P S Walsh; C H Levenson; H A Erlich
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205
  6 in total
  1 in total

1.  Systematic characterization of 2'-deoxynucleoside- 5'-triphosphate analogs as substrates for DNA polymerases by polymerase chain reaction and kinetic studies on enzymatic production of modified DNA.

Authors:  Masayasu Kuwahara; Jun-ichi Nagashima; Masatoshi Hasegawa; Takehiro Tamura; Rina Kitagata; Kazuo Hanawa; Shin-ichi Hososhima; Toshiyuki Kasamatsu; Hiroaki Ozaki; Hiroaki Sawai
Journal:  Nucleic Acids Res       Date:  2006-09-29       Impact factor: 16.971
  1 in total

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