Identification of a DNA segment in ferret Aleutian disease virus similar to a hypervariable capsid region of mink Aleutian disease parvovirus.

A 401bp DNA fragment of ferret Aleutian disease virus (ADV) was amplified using PCR primers spanning a hypervariable region of mink ADV capsid sequence. The amplified fragment was 88-89% homologous to the same region of previously known sequence of three different strains of mink ADV, however, as low as 54% homology was observed when compared with a 39bp segment known as hypervariable region. Within the predicted 13 amino acid hypervariable region, the ferret ADV sequence differed at 6 positions from the wild type mink Utah1 strain. Three amino acids Gln289, Glu293 and Thr295 in this region were common to the pathogenic ferret ADV, mink Utah1 and ADVK strains, but differed from the cell culture adapted nonpathogenic mink strain ADVG suggesting that these three conserved residues may have some functional significance.