Literature DB >> 8770418

Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells.

R G Chubet1, B L Brizzard.   

Abstract

The FLAG peptide, AspTyrLysAspAspAspAspLys, has been used as an epitope tag in a variety of cell types. The modification of the cytomegalovirus (CMV) promoter containing vector, pCMV5, to create two transient expression vectors designed for secretion and intracellular expression of FLAG-fusion proteins in mammalian cells is described. As a functional test, the bacterial alkaline phosphatase gene was cloned into both vectors, and anti-FLAG monoclonal antibodies were used for detection of FLAG epitope-tagged bacterial alkaline phosphatase in mammalian cells. In addition, secreted bacterial alkaline phosphatase was purified from the extracellular medium by anti-FLAG affinity chromatography.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8770418     DOI： 10.2144/96201pf01

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  16 in total

1.  Construction and application of epitope- and green fluorescent protein-tagging integration vectors for Bacillus subtilis.

Authors:  Marcus Kaltwasser; Thomas Wiegert; Wolfgang Schumann
Journal:  Appl Environ Microbiol       Date:  2002-05       Impact factor: 4.792

2.  Suppression of growth and migration by blocking the Hedgehog signaling pathway in gastric cancer cells.

Authors:  Runwei Yan; Xiang Peng; Xiaogang Yuan; Dengliang Huang; Jiang Chen; Quqin Lu; Nonghua Lv; Shiwen Luo
Journal:  Cell Oncol (Dordr)       Date:  2013-09-13       Impact factor: 6.730

3.  Coactivator recruitment is essential for liganded thyroid hormone receptor to initiate amphibian metamorphosis.

Authors:  Bindu Diana Paul; Liezhen Fu; Daniel R Buchholz; Yun-Bo Shi
Journal:  Mol Cell Biol       Date:  2005-07       Impact factor: 4.272

4.  Method for generation of in vivo biotinylated recombinant antibodies by yeast mating.

Authors:  Nathalie Scholler; Barbara Garvik; Travis Quarles; Shaoyi Jiang; Nicole Urban
Journal:  J Immunol Methods       Date:  2006-10-30       Impact factor: 2.303

5.  Geminivirus Replication Protein Impairs SUMO Conjugation of Proliferating Cellular Nuclear Antigen at Two Acceptor Sites.

Authors:  Manuel Arroyo-Mateos; Blanca Sabarit; Francesca Maio; Miguel A Sánchez-Durán; Tabata Rosas-Díaz; Marcel Prins; Javier Ruiz-Albert; Ana P Luna; Harrold A van den Burg; Eduardo R Bejarano
Journal:  J Virol       Date:  2018-08-29       Impact factor: 5.103

6.  Folding and subunit assembly of photoreceptor peripherin/rds is mediated by determinants within the extracellular/intradiskal EC2 domain: implications for heterogeneous molecular pathologies.

Authors:  A F Goldberg; L M Fales; J B Hurley; N Khattree
Journal:  J Biol Chem       Date:  2001-09-11       Impact factor: 5.157

7.  Modeling oculopharyngeal muscular dystrophy in myotube cultures reveals reduced accumulation of soluble mutant PABPN1 protein.

Authors:  Vered Raz; Samantha Routledge; Andrea Venema; Hellen Buijze; Erik van der Wal; Seyedyahya Anvar; Kirsten R Straasheijm; Rinse Klooster; Michael Antoniou; Silvère M van der Maarel
Journal:  Am J Pathol       Date:  2011-08-18       Impact factor: 4.307

8.  Immune-type receptor genes in zebrafish share genetic and functional properties with genes encoded by the mammalian leukocyte receptor cluster.

Authors:  J A Yoder; M G Mueller; S Wei; B C Corliss; D M Prather; T Willis; R T Litman; J Y Djeu; G W Litman
Journal:  Proc Natl Acad Sci U S A       Date:  2001-05-29       Impact factor: 11.205

9.  Tandem affinity purification of protein complexes in mouse embryonic stem cells using in vivo biotinylation.

Authors:  Jianlong Wang; Alan B Cantor; Stuart H Orkin
Journal:  Curr Protoc Stem Cell Biol       Date:  2009-03

10.  Characterization of the expression of Salmonella Type III secretion system factor PrgI, SipA, SipB, SopE2, SpaO, and SptP in cultures and in mice.

Authors:  Hao Gong; Jing Su; Yong Bai; Lu Miao; Kihoon Kim; Yonghua Yang; Fenyong Liu; Sangwei Lu
Journal:  BMC Microbiol       Date:  2009-04-17       Impact factor: 3.605
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.