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Literature DB >> 8770233

Measuring the viscoelastic properties of human platelets with the atomic force microscope.

M Radmacher¹, M Fritz, C M Kacher, J P Cleveland, P K Hansma.

Abstract

We have measured force curves as a function of the lateral position on top of human platelets with the atomic force microscope. These force curves show the indentation of the cell as the tip loads the sample. By analyzing these force curves we were able to determine the elastic modulus of the platelet with a lateral resolution of approximately 100 nm. The elastic moduli were in a range of 1-50 kPa measured in the frequency range of 1-50 Hz. Loading forces could be controlled with a resolution of 80 pN and indentations of the platelet could be determined with a resolution of 20 nm.

Entities: Gene Species

Mesh：

Year: 1996 PMID： 8770233 PMCID： PMC1224955 DOI： 10.1016/S0006-3495(96)79602-9

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

33 in total

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Authors: S Karrasch; R Hegerl; J H Hoh; W Baumeister; A Engel
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2. From molecules to cells: imaging soft samples with the atomic force microscope.

Authors: M Radmacher; R W Tillamnn; M Fritz; H E Gaub
Journal: Science Date: 1992-09-25 Impact factor: 47.728

3. Atomic force microscope.

Authors:
Journal: Phys Rev Lett Date: 1986-03-03 Impact factor: 9.161

4. Imaging viscoelasticity by force modulation with the atomic force microscope.

Authors: M Radmacher; R W Tillmann; H E Gaub
Journal: Biophys J Date: 1993-03 Impact factor: 4.033

5. The mechanical properties of actin gels. Elastic modulus and filament motions.

Authors: P A Janmey; S Hvidt; J Käs; D Lerche; A Maggs; E Sackmann; M Schliwa; T P Stossel
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6. Direct observation of enzyme activity with the atomic force microscope.

Authors: M Radmacher; M Fritz; H G Hansma; P K Hansma
Journal: Science Date: 1994-09-09 Impact factor: 47.728

7. Direct measurement of the forces between complementary strands of DNA.

Authors: G U Lee; L A Chrisey; R J Colton
Journal: Science Date: 1994-11-04 Impact factor: 47.728

8. Platelet activation and cytoskeletal reorganization: high voltage electron microscopic examination of intact and Triton-extracted whole mounts.

Authors: J C Loftus; J Choate; R M Albrecht
Journal: J Cell Biol Date: 1984-06 Impact factor: 10.539

9. Mechanics of fibroblast locomotion: quantitative analysis of forces and motions at the leading lamellas of fibroblasts.

Authors: S Felder; E L Elson
Journal: J Cell Biol Date: 1990-12 Impact factor: 10.539

10. Mechanisms of actin rearrangements mediating platelet activation.

Authors: J H Hartwig
Journal: J Cell Biol Date: 1992-09 Impact factor: 10.539

166 in total

1. High-resolution imaging of antibodies by tapping-mode atomic force microscopy: attractive and repulsive tip-sample interaction regimes.

Authors: A San Paulo; R García
Journal: Biophys J Date: 2000-03 Impact factor: 4.033

2. Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells.

Authors: A B Mathur; G A Truskey; W M Reichert
Journal: Biophys J Date: 2000-04 Impact factor: 4.033

Measuring the viscoelastic properties of human platelets with the atomic force microscope.

1. Atomic force microscopy produces faithful high-resolution images of protein surfaces in an aqueous environment.

2. From molecules to cells: imaging soft samples with the atomic force microscope.

3. Atomic force microscope.

4. Imaging viscoelasticity by force modulation with the atomic force microscope.

5. The mechanical properties of actin gels. Elastic modulus and filament motions.

6. Direct observation of enzyme activity with the atomic force microscope.

7. Direct measurement of the forces between complementary strands of DNA.

8. Platelet activation and cytoskeletal reorganization: high voltage electron microscopic examination of intact and Triton-extracted whole mounts.

9. Mechanics of fibroblast locomotion: quantitative analysis of forces and motions at the leading lamellas of fibroblasts.

10. Mechanisms of actin rearrangements mediating platelet activation.

1. High-resolution imaging of antibodies by tapping-mode atomic force microscopy: attractive and repulsive tip-sample interaction regimes.

2. Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells.

3. Mechanics of living cells measured by laser tracking microrheology.

4. Cell property determination from the acoustic microscope generated voltage versus frequency curves.

5. Direct characterization of the physicochemical properties of fungal spores using functionalized AFM probes.

6. Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study.

7. The optical stretcher: a novel laser tool to micromanipulate cells.

8. Lateral mechanical coupling of stereocilia in cochlear hair bundles.

9. Determination of elastic moduli of thin layers of soft material using the atomic force microscope.

10. Microrheology of human lung epithelial cells measured by atomic force microscopy.