Literature DB >> 8769606

High-resolution genotyping elucidates the epidemiology of group A streptococcus outbreaks.

J Stanley1, M Desai, J Xerry, A Tanna, A Efstratiou, R George.   

Abstract

Streptococcus pyogenes strains were genotyped by a combination of molecular methods for high- resolution epidemiologic studies of disease outbreaks. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the emm gene is reported. Alone or in conjunction with other molecular techniques (16S ribotyping, pulsed-field gel electrophoresis, and detection of exotoxin genes), PCR-RFLP could differentiate outbreak-related strains from contemporaneous background strains of the same M serotype. Three outbreaks were studied: pharyngitis in a boarding school (serotype M5), cross-infection in a hospital burn unit (serotype M76), and severe invasive disease in two elderly care homes (serotype R28). It was possible, for example, to identify within serotype R28 a clone with particular potential for invasive disease. In all cases, the four molecular methods yielded complementary results that were hierarchically related. Strains could be assigned to the outbreak or the background in a precise, reproducible, and rapid manner.

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Year:  1996        PMID: 8769606     DOI: 10.1093/infdis/174.3.500

Source DB:  PubMed          Journal:  J Infect Dis        ISSN: 0022-1899            Impact factor:   5.226


  13 in total

1.  High-resolution genotyping of Streptococcus pyogenes serotype M1 isolates by fluorescent amplified-fragment length polymorphism analysis.

Authors:  M Desai; A Efstratiou; R George; J Stanley
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

2.  Fluorescent amplified-fragment length polymorphism analysis of an outbreak of group A streptococcal invasive disease.

Authors:  M Desai; A Tanna; R Wall; A Efstratiou; R George; J Stanley
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948

3.  Group A streptococcal Vir types are M-protein gene (emm) sequence type specific.

Authors:  D L Gardiner; A M Goodfellow; D R Martin; K S Sriprakash
Journal:  J Clin Microbiol       Date:  1998-04       Impact factor: 5.948

4.  Genetic diversity among type emm28 group A Streptococcus strains causing invasive infections and pharyngitis.

Authors:  Nicole M Green; Stephen B Beres; Edward A Graviss; James E Allison; Allison J McGeer; Jaana Vuopio-Varkila; Rance B LeFebvre; James M Musser
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

5.  Characterization of antimicrobial resistance in Streptococcus pyogenes isolates from the San Francisco Bay area of northern California.

Authors:  M K York; L Gibbs; F Perdreau-Remington; G F Brooks
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

6.  Determination of the relationship between group A streptococcal genome content, M type, and toxic shock syndrome by a mixed genome microarray.

Authors:  Bart J M Vlaminckx; Frank H J Schuren; Roy C Montijn; Martien P M Caspers; Ad C Fluit; Wim J B Wannet; Leo M Schouls; Jan Verhoef; Wouter T M Jansen
Journal:  Infect Immun       Date:  2007-02-26       Impact factor: 3.441

7.  Genotyping of epidemic methicillin-resistant Staphylococcus aureus phage type 15 isolates by fluorescent amplified-fragment length polymorphism analysis.

Authors:  R Grady; M Desai; G O'Neill; B Cookson; J Stanley
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

8.  M.SpyI, a DNA methyltransferase encoded on a mefA chimeric element, modifies the genome of Streptococcus pyogenes.

Authors:  Chad W Euler; Patricia A Ryan; Judith M Martin; Vincent A Fischetti
Journal:  J Bacteriol       Date:  2006-11-03       Impact factor: 3.490

9.  Genetic diversity and exotoxin A production of group A streptococci causing sepsis.

Authors:  Eun Ha Koh; Nam Yong Lee; Eui Chong Kim; Sunjoo Kim
Journal:  J Korean Med Sci       Date:  2006-04       Impact factor: 2.153

10.  Group G streptococcal bacteremia in Jerusalem.

Authors:  Ronit Cohen-Poradosu; Joseph Jaffe; David Lavi; Sigal Grisariu-Greenzaid; Ran Nir-Paz; Lea Valinsky; Mary Dan-Goor; Colin Block; Bernard Beall; Allon E Moses
Journal:  Emerg Infect Dis       Date:  2004-08       Impact factor: 6.883

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