Literature DB >> 8769308

Immunolocalization and pharmacological relevance of oligopeptide transporter PepT1 in intestinal absorption of beta-lactam antibiotics.

Y Sai1, I Tamai, H Sumikawa, K Hayashi, T Nakanishi, O Amano, M Numata, S Iseki, A Tsuji.   

Abstract

A polyclonal antibody (anti-PepT1/C) was raised against the rabbit intestinal H(+)-coupled oligopeptide transporter, PepT1. Anti-PepT1/C detected 70-80-kDa protein in crude membranes obtained from rabbit duodenum, jejunum and ileum. PepT1 was localized in the brush-border of the absorptive epithelial cells by subcellular fractionation of membranes on a sucrose density gradient and by immunohistochemistry using light and electron microscopy. Transport activity for cephalosporins and dipeptide expressed in Xenopus laevis oocytes injected with total mRNA obtained from rabbit small intestine was eliminated completely by prehybridization of the mRNA with antisense oligonucleotide against the 5'-coding region of rabbit PepT1 cDNA.

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Year:  1996        PMID： 8769308     DOI： 10.1016/0014-5793(96)00778-8

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  12 in total

1.  Function and immunolocalization of overexpressed human intestinal H+/peptide cotransporter in adenovirus-transduced Caco-2 cells.

Authors:  C P Hsu; E Walter; H P Merkle; B Rothen-Rutishauser; H Wunderli-Allenspach; J M Hilfinger; G L Amidon
Journal:  AAPS PharmSci       Date:  1999

Review 2.  Bile acid transporter-mediated oral drug delivery.

Authors:  Feiyang Deng; You Han Bae
Journal:  J Control Release       Date:  2020-07-22       Impact factor: 9.776

3.  Development and utility of anti-PepT1 anti-peptide polyclonal antibodies.

Authors:  S K Basu; J Shen; K J Elbert; C T Okamoto; V H Lee; H von Grafenstein
Journal:  Pharm Res       Date:  1998-02       Impact factor: 4.200

4.  PepT1 mRNA expression is induced by starvation and its level correlates with absorptive transport of cefadroxil longitudinally in the rat intestine.

Authors:  Kazumasa Naruhashi; Yoshimichi Sai; Ikumi Tamai; Nagao Suzuki; Akira Tsuji
Journal:  Pharm Res       Date:  2002-10       Impact factor: 4.200

5.  The extracellular calcium-sensing receptor is required for cholecystokinin secretion in response to L-phenylalanine in acutely isolated intestinal I cells.

Authors:  Alice P Liou; Yoshitatsu Sei; Xilin Zhao; Jianying Feng; Xinping Lu; Craig Thomas; Susanne Pechhold; Helen E Raybould; Stephen A Wank
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2011-01-20       Impact factor: 4.052

6.  PepT1 mediates transport of the proinflammatory bacterial tripeptide L-Ala-{gamma}-D-Glu-meso-DAP in intestinal epithelial cells.

Authors:  Guillaume Dalmasso; Hang Thi Thu Nguyen; Laetitia Charrier-Hisamuddin; Yutao Yan; Hamed Laroui; Benjamin Demoulin; Shanthi V Sitaraman; Didier Merlin
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2010-06-17       Impact factor: 4.052

7.  PepT1-mediated tripeptide KPV uptake reduces intestinal inflammation.

Authors:  Guillaume Dalmasso; Laetitia Charrier-Hisamuddin; Hang Thi Thu Nguyen; Yutao Yan; Shanthi Sitaraman; Didier Merlin
Journal:  Gastroenterology       Date:  2007-10-17       Impact factor: 22.682

8.  Expression of the peptide transporter hPepT1 in human colon: a potential route for colonic protein nitrogen and drug absorption.

Authors:  Dianne Ford; Alison Howard; Barry H Hirst
Journal:  Histochem Cell Biol       Date:  2002-12-10       Impact factor: 4.304

9.  Epigenetic regulation of intestinal peptide transporter PEPT1 as a potential strategy for colorectal cancer sensitization.

Authors:  Yanhong Wang; Jiaqi Wang; Lingrong Yang; Liqing Qiu; Yuhui Hua; Shixiu Wu; Su Zeng; Lushan Yu; Xiaoli Zheng
Journal:  Cell Death Dis       Date:  2021-05-24       Impact factor: 8.469

10.  Characterization of the uptake mechanism for a novel loop diuretic, M17055, in Caco-2 cells: involvement of organic anion transporting polypeptide (OATP)-B.

Authors:  Tomohiro Nishimura; Yoshiyuki Kubo; Yukio Kato; Yoshimichi Sai; Takuo Ogihara; Akira Tsuji
Journal:  Pharm Res       Date:  2006-11-14       Impact factor: 4.580
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