Literature DB >> 8768258

Characterization of human, mouse and rabbit anti-gliadin antibodies by ELISA and western blotting.

Z Vancíková1, P Kocna, L Tucková, P Fric, M Dvorák, S Stoyanov, H Tlaskalová-Hogenová.   

Abstract

Monoclonal, hyperimmune rabbit and human serum anti-gliadin antibodies were analyzed by ELISA and immunoblotting techniques. In Western blotting the difference in reactivity between monoclonal and human antibodies was quantitative rather than qualitative. Rabbit antisera differed in reactivity according to the protein used for immunization. The rabbits immunized by the peptic-tryptic pancreatic digest of gliadin reacted similarly to the patients. In ELISA, significantly higher reactivity with crude, A-, glyc-gli, alpha-, beta- and omega-gliadins was found in the patients' sera than in controls.

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Year:  1995        PMID： 8768258     DOI： 10.1007/bf02818526

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  18 in total

1.  Gluten-induced enteropathy: the effect of partially digested gluten.

Authors:  A C FRAZER; R F FLETCHER; C A ROSS; B SHAW; H G SAMMONS; R SCHNEIDER
Journal:  Lancet       Date:  1959-09-05       Impact factor: 79.321

2.  Relationship between gliadin peptide structure and their effect on the fetal chick duodenum.

Authors:  P Kocna; T Mothes; V Krchnák; P Fric
Journal:  Z Lebensm Unters Forsch       Date:  1991-02

Review 3.  Gluten, major histocompatibility complex, and the small intestine. A molecular and immunobiologic approach to the spectrum of gluten sensitivity ('celiac sprue').

Authors:  M N Marsh
Journal:  Gastroenterology       Date:  1992-01       Impact factor: 22.682

4.  Isolation and analysis of peptidic fragments of alpha-gliadin using reversed-phase high-performance liquid chromatography.

Authors:  P Kocna; P Fric; M Kocová-Holáková; J Slabý; E Kasafírek; W T Hekkens
Journal:  J Chromatogr       Date:  1988-12-30

5.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Immunoblotting analysis of antigliadin antibodies in the sera of patients with dermatitis herpetiformis and gluten-sensitive enteropathy.

Authors:  E Vainio
Journal:  Int Arch Allergy Appl Immunol       Date:  1986

7.  Molecular mimicry as a possible cause of autoimmune reactions in celiac disease? Antibodies to gliadin cross-react with epitopes on enterocytes.

Authors:  L Tucková; H Tlaskalová-Hogenová; M A Farré; K Karská; P Rossmann; J Kolínská; P Kocna
Journal:  Clin Immunol Immunopathol       Date:  1995-02

8.  Maturation of the head of bacteriophage T4. I. DNA packaging events.

Authors:  U K Laemmli; M Favre
Journal:  J Mol Biol       Date:  1973-11-15       Impact factor: 5.469

9.  Polyproline, beta-turn helices. Novel secondary structures proposed for the tandem repeats within rhodopsin, synaptophysin, synexin, gliadin, RNA polymerase II, hordein, and gluten.

Authors:  N Matsushima; C E Creutz; R H Kretsinger
Journal:  Proteins       Date:  1990

10.  Calreticulin--the potential autoantigen in celiac disease.

Authors:  K Karská; L Tucková; L Steiner; H Tlaskalová-Hogenová; M Michalak
Journal:  Biochem Biophys Res Commun       Date:  1995-04-17       Impact factor: 3.575
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  1 in total

1.  A curated gluten protein sequence database to support development of proteomics methods for determination of gluten in gluten-free foods.

Authors:  Sophie Bromilow; Lee A Gethings; Mike Buckley; Mike Bromley; Peter R Shewry; James I Langridge; E N Clare Mills
Journal:  J Proteomics       Date:  2017-04-04       Impact factor: 4.044
  1 in total

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