Literature DB >> 8761736

Characterization of enterotoxigenic Escherichia coli by random amplification of polymorphic DNA.

A B Pacheco1, B E Guth, D F de Almeida, L C Ferreira.   

Abstract

Two enterotoxigenic Escherichia coli (ETEC) strains (H10407 and 4011-1) were characterized by random amplification of polymorphic DNA (RAPD) profiles using 10-mer oligonucleotides with diverse GC content. All tested primers yielded arrays of amplified DNA products ranging in size from 200 to 3000 bp. The effects of annealing temperature, template concentration and GC content of the primers were evaluated and an optimal reaction procedure was established. Application of the RAPD analysis to ten ETEC strains belonging to five different serotypes showed that strains of the same serotype shared identical or almost identical band profiles, suggesting a similar genetic composition. The use of RAPD profiles as a tool in epidemiological analysis of ETEC is discussed.

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Year:  1996        PMID： 8761736     DOI： 10.1016/0923-2508(96)80217-8

Source DB:  PubMed          Journal:  Res Microbiol        ISSN： 0923-2508            Impact factor:   3.992


  12 in total

1.  Osmotic stress-induced genetic rearrangements in Escherichia coli H10407 detected by randomly amplified polymorphic DNA analysis.

Authors:  A Jolivet-Gougeon; S David-Jobert; Z Tamanai-Shacoori; C Ménard; M Cormier
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

2.  Detection of qnr in clinical isolates of Escherichia coli from Korea.

Authors:  Jin-Yong Jeong; Hyun Jung Yoon; Eun Sil Kim; Yoola Lee; Sang-Ho Choi; Nam Joong Kim; Jun Hee Woo; Yang Soo Kim
Journal:  Antimicrob Agents Chemother       Date:  2005-06       Impact factor: 5.191

3.  Molecular characterization of Escherichia coli strains that cause symptomatic and asymptomatic urinary tract infections.

Authors:  Sam Abraham; Toni A Chapman; Ren Zhang; James Chin; Amanda N Mabbett; Makrina Totsika; Mark A Schembri
Journal:  J Clin Microbiol       Date:  2011-12-21       Impact factor: 5.948

4.  Random amplification of polymorphic DNA reveals serotype-specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans.

Authors:  A B Pacheco; B E Guth; K C Soares; L Nishimura; D F de Almeida; L C Ferreira
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

5.  Genetic diversity of Cercospora kikuchii isolates from soybean cultured in Argentina as revealed by molecular markers and cercosporin production.

Authors:  María Cristina Lurá; María Gabriela Latorre Rapela; María Celia Vaccari; Roxana Maumary; Anabel Soldano; Mónica Mattio; Ana María González
Journal:  Mycopathologia       Date:  2010-09-12       Impact factor: 2.574

6.  Escherichia coli ehl1 gene-positive serotype O18ac:H31 associated with an outbreak of diarrhea in a neonatal nursery in Neuquén City, Argentina.

Authors:  Isabel Chinen; Marta Rivas; Viviana Soriano; Elizabeth Miliwebsky; Gabriel Fernández Galvez; Germán Chillemi; Ariela Baschkier; Gehua Wang; Richard Caldeira; David L Woodward; Frank G Rodgers
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

Review 7.  Occurrence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli.

Authors:  M K Wolf
Journal:  Clin Microbiol Rev       Date:  1997-10       Impact factor: 26.132

8.  Origin of contamination and genetic diversity of Escherichia coli in beef cattle.

Authors:  Mueen Aslam; Frances Nattress; Gordon Greer; Chris Yost; Colin Gill; Lynn McMullen
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

9.  Genetic diversity of heat-labile toxin expressed by enterotoxigenic Escherichia coli strains isolated from humans.

Authors:  M A Lasaro; J F Rodrigues; C Mathias-Santos; B E C Guth; A Balan; M E Sbrogio-Almeida; L C S Ferreira
Journal:  J Bacteriol       Date:  2008-01-25       Impact factor: 3.490

10.  Molecular characterization of commensal Escherichia coli adapted to different compartments of the porcine gastrointestinal tract.

Authors:  Sam Abraham; David M Gordon; James Chin; Huub J M Brouwers; Peter Njuguna; Mitchell D Groves; Ren Zhang; Toni A Chapman
Journal:  Appl Environ Microbiol       Date:  2012-07-13       Impact factor: 4.792
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