Literature DB >> 8760903

Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines.

J H Boal1, A Wilk, N Harindranath, E E Max, T Kempe, S L Beaucage.   

Abstract

A novel method for the deprotection of oligodeoxyribonucleotides has been developed. Gaseous amines such as ammonia or methylamine were employed under pressure to achieve mild and rapid deprotection conditions. For example, oligodeoxyribonucleotides having a (tert-butyl)phenoxyacetyl group for the protection of the exocyclic amino function of cytosine, adenine and guanine were released from controlled-pore glass supports and fully deprotected by ammonia or methylamine under gas phase conditions, at room temperature, within 35 or 2 min respectively.

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Year:  1996        PMID： 8760903      PMCID： PMC146024          DOI： 10.1093/nar/24.15.3115

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  5 in total

1.  Oligodeoxyribonucleotide phosphotriesters.

Authors:  M Koziolkiewicz; A Wilk
Journal:  Methods Mol Biol       Date:  1993

2.  A survey of nucleic acid services in core laboratories.

Authors:  R T Pon; G A Buck; R L Niece; M Robertson; A J Smith; E Spicer
Journal:  Biotechniques       Date:  1994-09       Impact factor: 1.993

3.  Labile exocyclic amine protection of nucleosides in DNA, RNA and oligonucleotide analog synthesis facilitating N-deacylation, minimizing depurination and chain degradation.

Authors:  N D Sinha; P Davis; N Usman; J Pérez; R Hodge; J Kremsky; R Casale
Journal:  Biochimie       Date:  1993       Impact factor: 4.079

4.  The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups.

Authors:  J C Schulhof; D Molko; R Teoule
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971

5.  The isopropoxyacetic group for convenient base protection during solid-support synthesis of oligodeoxyribonucleotides and their triester analogs.

Authors:  B Uznanski; A Grajkowski; A Wilk
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971
  5 in total
  7 in total

1.  Hydroquinone-O,O'-diacetic acid ('Q-linker') as a replacement for succinyl and oxalyl linker arms in solid phase oligonucleotide synthesis.

Authors:  R T Pon; S Yu
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

2.  Gas-phase cleavage and dephosphorylation of universal linker-bound oligodeoxynucleotides.

Authors:  Michael A Jensen; Keith M Anderson; Ronald W Davis
Journal:  Nucleosides Nucleotides Nucleic Acids       Date:  2010-11       Impact factor: 1.381

3.  Microwave-assisted rapid deprotection of oligodeoxyribonucleotides.

Authors:  P Kumar; K C Gupta
Journal:  Nucleic Acids Res       Date:  1997-12-15       Impact factor: 16.971

4.  High throughput parallel synthesis of oligonucleotides with 1536 channel synthesizer.

Authors:  Ji-Yen Cheng; Hung-Hui Chen; Yu-Shu Kao; Wei-Chen Kao; Konan Peck
Journal:  Nucleic Acids Res       Date:  2002-09-15       Impact factor: 16.971

5.  Thermolytic CpG-containing DNA oligonucleotides as potential immunotherapeutic prodrugs.

Authors:  Andrzej Grajkowski; Joao Pedras-Vasconcelos; Vivian Wang; Cristina Ausín; Sonja Hess; Daniela Verthelyi; Serge L Beaucage
Journal:  Nucleic Acids Res       Date:  2005-06-21       Impact factor: 16.971

6.  Synthesis of oligodeoxynucleotides using fully protected deoxynucleoside 3'-phosphoramidite building blocks and base recognition of oligodeoxynucleotides incorporating N3-cyano-ethylthymine.

Authors:  Hirosuke Tsunoda; Tomomi Kudo; Akihiro Ohkubo; Kohji Seio; Mitsuo Sekine
Journal:  Molecules       Date:  2010-10-27       Impact factor: 4.411

7.  Derivatization of DNAs with selenium at 6-position of guanine for function and crystal structure studies.

Authors:  Jozef Salon; Jiansheng Jiang; Jia Sheng; Oksana O Gerlits; Zhen Huang
Journal:  Nucleic Acids Res       Date:  2008-11-05       Impact factor: 16.971
  7 in total

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