Literature DB >> 876029

Extrachromosomal inheritance in Schizosaccharomyces pombe. III. Isolation and characterization of paromomycin-resistant mutants.

L Del Giudice, K Wolf, G Seitz, G Burger, B Lang, F Kaudewitz.   

Abstract

In the antimycin--resistant mutant anar-8 of the fission yeast Schizosaccharomyces pombe (Sch.p.) spontaneous mutants were isolated showing high resistance to the aminoglycoside antibiotic paromomycin. All mutants were resistant to the structurally related antibiotic neomycin. Tetrad analysis, mitotic segregation analysis, and mitotic haploidization revealed extrachromosomal, very likely mitochondrial inheritance. In contrast to the rapid segregation of mitochondrial markers in zygotic clones of Saccharomyces cerevisiae (S.c.) the heteroplasmic state of diploids proved to persist for at least 50 generations after zygote formation. Stationary cultures of the paromomycin-resistant mutants parr-106 and parr-112 contain up to 6% respiratory-deficient mutants, but no reversion to paromomycin-sensitivity was observed among 1700-1800 colonies tested. The ability of mutant anar-8 to produce spontaneously respiratory-deficient mutants could be separated from the antimycin-resistant phenotype of anar-8.

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Year:  1977        PMID： 876029     DOI： 10.1007/bf00693086

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  13 in total

1.  Studies on the mechanism of electron trasport in the bc1-segment of the respiratory chain in yeast. III. Isolation and characterization of an antimycin resistant mutant ANT 8 in Schizosaccharomyces pombe.

Authors:  B Lang; G Burger; K Wolf; W Bandlow; F Kaudewitz
Journal:  Mol Gen Genet       Date:  1975

Review 2.  Inhibitors of protein synthesis.

Authors:  D Vazquez
Journal:  FEBS Lett       Date:  1974-03-23       Impact factor: 4.124

3.  Mitochondrial genetics. V. Multifactorial mitochondrial crosses involving a mutation conferring paromomycin-resistance in Saccharomyces cerevisiae.

Authors:  K Wolf; B Dujon; P P Slonimski
Journal:  Mol Gen Genet       Date:  1973-09-05

4.  Respiration deficient mutants of Schizosaccharomyces Pombe I.

Authors:  K Wolf; M Sebald-Althaus; R J Schweyen; F Kaudewitz
Journal:  Mol Gen Genet       Date:  1971

5.  Biogenesis of mitochondria. 12. The effects of aminoglycoside antibiotics on the mitochondrial and cytoplasmic protein-synthesizing systems of Saccharomyces cerevisiae.

Authors:  P J Davey; J M Haslam; A W Linnane
Journal:  Arch Biochem Biophys       Date:  1970-01       Impact factor: 4.013

6.  Recombination of mitochondrial drug-resistance factors in Saccharomyces cerevisiae.

Authors:  D Y Thomas; D Wilkie
Journal:  Biochem Biophys Res Commun       Date:  1968-02-26       Impact factor: 3.575

7.  The biogenesis of mitochondria. V. Cytoplasmic inheritance of erythromycin resistance in Saccharomyces cerevisiae.

Authors:  A W Linnane; G W Saunders; E B Gingold; H B Lukins
Journal:  Proc Natl Acad Sci U S A       Date:  1968-03       Impact factor: 11.205

8.  Mapping of mitochondrial genes in Saccharomyces cerevisiae. Populations and pedigree analysis of retention or loss of four genetic markers in Rho-cells.

Authors:  R J Schweyen; U Steyrer; F Kaudewitz
Journal:  Mol Gen Genet       Date:  1976-07-23

9.  Extrachromosomal inheritance in Schizosaccharomyces pombe. II. Evidence for extrakaryotically inherited respiratory deficient mutants.

Authors:  K Wolf; B Lang; G Burger; F Kaudewitz
Journal:  Mol Gen Genet       Date:  1976-02-27

10.  Recombination among three mitochondrial genes in yeast (Saccharomyces cerevisiae).

Authors:  R A Kleese; R C Grotbeck; J R Snyder
Journal:  J Bacteriol       Date:  1972-11       Impact factor: 3.490
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  7 in total

1.  Protoplast fusion in a petite-negative yeast, Kluyveromyces lactis.

Authors:  A J Morgan; A Brunner; P A Whittaker
Journal:  Curr Genet       Date:  1980-07       Impact factor: 3.886

2.  The mitochondrial genome of the fission yeast Schizosaccharomyces pombe : 5. Characterization of mitochondrial deletion mutants.

Authors:  F Ahne; A M Merlos-Lange; B F Lang; K Wolf
Journal:  Curr Genet       Date:  1984-09       Impact factor: 3.886

3.  Extrachromosomal inheritance in Schizosaccharomyces pombe. VIII. Extent of cytoplasmic mixing in zygotes estimated by tetrad analysis of crosses involving mitochondrial markers conferring resistance to antimycin, chloramphenicol, and erythromycin.

Authors:  K Wolf; G Seitz-Mayr; F Kaudewitz
Journal:  Mol Gen Genet       Date:  1978-09-08

4.  Extrachromosomal inheritance in Schizosaccharomyces pombe. VII. Studies by zygote clone analysis on transmission, segregation, recombination, and uniparental inheritance of mitochondrial markers conferring resistance to antimycin, chloramphenicol, and erythromycin.

Authors:  G Seitz-Mayr; K Wolf; F Kaudewitz
Journal:  Mol Gen Genet       Date:  1978-09-08

5.  Promotion of uniparental inheritance of mitochondrial drug resistance by delayed division of yeast zygotes.

Authors:  K Wolf; L Del Giudice; F Kaudewitz
Journal:  Mol Gen Genet       Date:  1979-10-03

Review 6.  Gene database for the fission yeast Schizosaccharomyces pombe.

Authors:  G G Lennon; H Lehrach
Journal:  Curr Genet       Date:  1992-01       Impact factor: 3.886

7.  Extrachromosomal inheritance in Schizosaccharomyces pombe. IV. Isolation and genetic characterization of mutants resistant to chloramphenicol and erythromycin using the mutator properties of mutant anar-8.

Authors:  G Seitz; K Wolf; F Kaudewitz
Journal:  Mol Gen Genet       Date:  1977-10-24
  7 in total

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