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Literature DB >> 876024

The isolation and genetic characterization of extrachromosomal chloramphenicol and oligomycin-resistant mutants from the petite-negative yeast Kluyveromyces lactis.

Abstract

Spontaneous mutants of the petite-negative yeast Kluyveromyces lactis, resistant to the antibiotics chloramphenicol and oligomycin, were isolated and genetically characterized. Three chloramphenicol-resistant mutants showed non-Mendelian inheritance when crossed to sensitive parents. Of 5 oligomycin-resistant strains studied, three exhibited resistance due to the presence of an extrachromosomal mutation. The resistance of the other two deriving from a nuclear and recessive mutation. When two factor crosses in trans configuration were performed between two of the chloramphenicol and the five oligomycin-resistant mutants a polarity in recombination was observed with a predominance of sensitive (OSCS) over resistant (ORCR) reciprocal recombinants. Allelism tests carried out among the oligomycin-resistant mutants indicated the presence of at least two distinct extrachromosomal regions responsible for the resistance.

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Year: 1977 PMID： 876024 DOI： 10.1007/bf00268816

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

18 in total

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Authors: C J BULDER
Journal: Antonie Van Leeuwenhoek Date: 1964 Impact factor: 2.271

2. ANTIBIOTIC STUDIES. II. INHIBITION OF PHOSPHORYL TRANSFER IN MITOCHONDRIA BY OLIGOMYCIN AND AUROVERTIN.

Authors: H A LARDY; J L CONNELLY; D JOHNSON
Journal: Biochemistry Date: 1964-12 Impact factor: 3.162

3. LETHALITY OF THE PETITE MUTATION IN PETITE NEGATIVE YEASTS.

Authors: C J BULDER
Journal: Antonie Van Leeuwenhoek Date: 1964 Impact factor: 2.271

4. The dissociation of phenotypic and inheritable effects of euflavin in yeast.

Authors: R H DE DEKEN
Journal: Exp Cell Res Date: 1961-06 Impact factor: 3.905

5. Mitochondrial genetics. V. Multifactorial mitochondrial crosses involving a mutation conferring paromomycin-resistance in Saccharomyces cerevisiae.

Authors: K Wolf; B Dujon; P P Slonimski
Journal: Mol Gen Genet Date: 1973-09-05

6. Cytoplasmic and nuclear inheritance of resistance to alkylguanidines and ethidium bromide in a petite-negative yeast.

Authors: A Brunner; J Mas; E Celis; J R Mattoon
Journal: Biochem Biophys Res Commun Date: 1973-07-17 Impact factor: 3.575

7. Mitochondrial DNA. V. A 25 micron closed circular duplex DNA molecule in wild-type yeast mitochondria. Stucture and genetic complexity.

Authors: C P Hollenberg; P Borst; E F van Bruggen
Journal: Biochim Biophys Acta Date: 1970-05-21

3. Genetic instability of an oligomycin resistance mutation in yeast is associated with an amplification of a mitochondrial DNA segment.

Authors: A Ragnini; H Fukuhara
Journal: Nucleic Acids Res Date: 1989-09-12 Impact factor: 16.971

4. Mitochondrial Four-Point Crosses in ASPERGILLUS NIDULANS : Mapping of a Suppressor of a Mitochondrially Inherited Cold-Sensitive Mutation.

Authors: R B Waring; C Scazzocchio
Journal: Genetics Date: 1983-03 Impact factor: 4.562

5. Extrachromosomal inheritance in Schizosaccharomyces pombe. VII. Studies by zygote clone analysis on transmission, segregation, recombination, and uniparental inheritance of mitochondrial markers conferring resistance to antimycin, chloramphenicol, and erythromycin.

Authors: G Seitz-Mayr; K Wolf; F Kaudewitz
Journal: Mol Gen Genet Date: 1978-09-08

6. Isolation of chloramphenicol-resistant mutants of Kluyveromyces lactis and characterization by mitotic segregation analysis of fused hybrids.

Authors: B M Allmark
Journal: Antonie Van Leeuwenhoek Date: 1984 Impact factor: 2.271

7. Extrachromosomal oligomycin-resistant mutants of the petite-negative yeast Kluyveromyces lactis. Properties of mitochondrial ATPase and cross-resistance to inhibitors of phosphoryl transfer reactions.

Authors: A Brunner; A Tuena de Cobos
Journal: Mol Gen Genet Date: 1980

8. Glucose repression of lactose/galactose metabolism in Kluyveromyces lactis is determined by the concentration of the transcriptional activator LAC9 (K1GAL4) [corrected].

Authors: W Zachariae; P Kuger; K D Breunig
Journal: Nucleic Acids Res Date: 1993-01-11 Impact factor: 16.971

9. Extrachromosomal inheritance in Schizosaccharomyces pombe. IV. Isolation and genetic characterization of mutants resistant to chloramphenicol and erythromycin using the mutator properties of mutant anar-8.

Authors: G Seitz; K Wolf; F Kaudewitz
Journal: Mol Gen Genet Date: 1977-10-24

10. Functional homology between the yeast regulatory proteins GAL4 and LAC9: LAC9-mediated transcriptional activation in Kluyveromyces lactis involves protein binding to a regulatory sequence homologous to the GAL4 protein-binding site.

Authors: K D Breunig; P Kuger
Journal: Mol Cell Biol Date: 1987-12 Impact factor: 4.272