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Literature DB >> 876023

Rapid screening for plasmid DNA.

Abstract

A procedure is described for demonstrating plasmid DNA and its molecular weight, based on rate zonal centrifugation of unlabelled DNA in neutral sucrose gradients containing a low concentration of ethidium bromide. Each DNA species is then visualized as a discrete fluorescent band when the centrifuge tube is illuminated with ultra-violet light. Plasmids exist as closed circular and as relaxed circular molecules, which sediment separately, but during preparation of lysates, closed circular molecules are nicked so that each plasmid forms only a single band of relaxed circles within the gradient.

Entities: Chemical

Mesh：

Substances：
DNA, Bacterial
Ethidium

Year: 1977 PMID： 876023 DOI： 10.1007/bf00338692

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

19 in total

1. Sedimentation rate as a measure of molecular weight of DNA.

Authors: E BURGI; A D HERSHEY
Journal: Biophys J Date: 1963-07 Impact factor: 4.033

2. Two major groups of colicin factors: their evolutionary significance.

Authors: K G Hardy; G G Meynell; J E Dowman; B G Spratt
Journal: Mol Gen Genet Date: 1973-09-12

3. General method for the isolation of plasmid deoxyribonucleic acid.

Authors: P Guerry; D J LeBlanc; S Falkow
Journal: J Bacteriol Date: 1973-11 Impact factor: 3.490

4. The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

Authors: W Bauer; J Vinograd
Journal: J Mol Biol Date: 1968-04-14 Impact factor: 5.469

5. The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex.

Authors: O G Stonington; D E Pettijohn
Journal: Proc Natl Acad Sci U S A Date: 1971-01 Impact factor: 11.205

6. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

7. Simplified quantitative electron microscopy of biopolymers.

Authors: D Lang; M Mitani
Journal: Biopolymers Date: 1970 Impact factor: 2.505

8. Problems in the extraction DNA when utilizing pancreatic RNAase and pronase.

Authors: J J McCormick; L J Larson; V M Maher
Journal: Biochim Biophys Acta Date: 1974-05-17

9. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

Authors: J A Meyers; D Sanchez; L P Elwell; S Falkow
Journal: J Bacteriol Date: 1976-09 Impact factor: 3.490

10. Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control.

Authors: P Kontomichalou; M Mitani; R C Clowes
Journal: J Bacteriol Date: 1970-10 Impact factor: 3.490

9 in total

Rapid screening for plasmid DNA.

1. Sedimentation rate as a measure of molecular weight of DNA.

2. Two major groups of colicin factors: their evolutionary significance.

3. General method for the isolation of plasmid deoxyribonucleic acid.

4. The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye.

5. The folded genome of Escherichia coli isolated in a protein-DNA-RNA complex.

6. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

7. Simplified quantitative electron microscopy of biopolymers.

8. Problems in the extraction DNA when utilizing pancreatic RNAase and pronase.

9. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid.

10. Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control.

1. Isolation of Plasmid DNA from Butyrivibrio fibrisolvens.

2. Organization of rRNA genes in Mycobacterium bovis BCG.

3. Physical map of the recA gene.

4. Two major groups of colicin factors: their molecular weights.

5. Requirements for suppression of a dnaG mutation by an I-type plasmid.

6. Complete nucleotide sequence of the 16S rRNA gene of Mycobacterium bovis BCG.

7. Sequence of the Saccharomyces cerevisiae PHR1 gene and homology of the PHR1 photolyase to E. coli photolyase.

8. Transfer of nitrogen fixation genes into Pseudomonas putida isolated from Finnish tundra soil.

9. Molecular cloning and characterization of an rRNA operon in Streptomyces lividans TK21.