Literature DB >> 875889

Radioimmunoassay for LCM virus antigens and anti-LCM virus antibodies and its application in an epidemiologic survey of people exposed to syrian hamsters.

M Blechschmidt, W Gerlich, R Thomssen.   

Abstract

A specific and sensitive solid-phase radioimmunoassay has been developed for the detection of LCM virus antigens and anti-LCM virus antibodies. The test was performed in a microtiter system using polyvinylcholoride wells coated with anti-LCM virus rabbit hyperimmune serum. LCM virus antigens were allowed to bind to this antibody and afterwards detected by 125J-labeled anti-LCM virus-gamma-globulin. Anti-LCM virus antibodies were assayed by specific inhibition of these bound antigens. Since this technique is rapid and easy to perform the solid-phase radioimmunoassay is a valuable test for detecting LCM virus infections. Selected at random, 208 girls with, and 208 girls without, contact to Syrian hamsters were investigated for anti-LCM virus antibodies. Five (2.4%) of the hamster-exposed girls had anti-LCM virus antibodies (RIA-titer 1:8-1:32) in contrast to none of the control group (P=0.03).

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Year:  1977        PMID： 875889     DOI： 10.1007/bf02126711

Source DB:  PubMed          Journal:  Med Microbiol Immunol        ISSN： 0300-8584            Impact factor:   3.402


  19 in total

1.  [A CHROMATOGRAPHIC PROCEDURE FOR THE DETERMINATION OF TYPE-SPECIFIC POLIOVIRUS ANTIBODIES WITH P32-LABELLED POLIOVIRUS].

Authors:  R THOMSSEN
Journal:  Z Naturforsch B       Date:  1963-10       Impact factor: 1.047

2.  Preparation of iodine-131 labelled human growth hormone of high specific activity.

Authors:  W M HUNTER; F C GREENWOOD
Journal:  Nature       Date:  1962-05-05       Impact factor: 49.962

3.  The hamster as a secondary reservoir host of lymphocytic choriomeningitis virus.

Authors:  H H Skinner; E H Knight; L S Buckley
Journal:  J Hyg (Lond)       Date:  1976-04

4.  Determination of protein: a modification of the Lowry method that gives a linear photometric response.

Authors:  E F Hartree
Journal:  Anal Biochem       Date:  1972-08       Impact factor: 3.365

5.  [Syrian hamsters as vectors of lymphocytic choriomeningitis].

Authors:  R Ackermann; W Stille; W Blumenthal; E B Helm; K Keller; O Baldus
Journal:  Dtsch Med Wochenschr       Date:  1972-11-10       Impact factor: 0.628

6.  Lymphocytic choriomeningitis virus in man. Serologic evidence of association with pet hamsters;.

Authors:  R Deibel; J P Woodall; W J Decher; G D Schryver
Journal:  JAMA       Date:  1975-05-05       Impact factor: 56.272

7.  Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125 I-antibody.

Authors:  C M Ling; L R Overby
Journal:  J Immunol       Date:  1972-10       Impact factor: 5.422

8.  The diagnostical significance of antibodies against hepatitis B core antigen.

Authors:  W H Gerlich; R M Biswas; B Stamm; R Thomssen
Journal:  Klin Wochenschr       Date:  1977-11-01

9.  Lymphocytic choriomeningitis outbreak associated with pet hamsters. Fifty-seven cases from New York State;.

Authors:  R J Biggar; J P Woodall; P D Walter; G E Haughie
Journal:  JAMA       Date:  1975-05-05       Impact factor: 56.272

10.  A SOLUBLE ANTIGEN OF LYMPHOCYTIC CHORIOMENINGITIS : II. INDEPENDENCE OF ANTI-SOLUBLE SUBSTANCE ANTIBODIES AND NEUTRALIZING ANTIBODIES, AND THE ROLE OF SOLUBLE ANTIGEN AND INACTIVE VIRUS IN IMMUNITY TO INFECTION.

Authors:  J E Smadel; M J Wall
Journal:  J Exp Med       Date:  1940-09-30       Impact factor: 14.307
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  5 in total

Review 1.  Ambisense RNA genomes of arenaviruses and phleboviruses.

Authors:  D H Bishop
Journal:  Adv Virus Res       Date:  1986       Impact factor: 9.937

2.  Sensitive solid-phase radioimmunoassay for detection of human immunoglobulin G antibodies to varicella-zoster virus.

Authors:  M G Friedman; S Leventon-Kriss; I Sarov
Journal:  J Clin Microbiol       Date:  1979-01       Impact factor: 5.948

3.  [A serological study concerning the role of the golden hamster (Mesocricetus auratus) in transmitting lymphocytic choriomeningitis virus to humans (author's transl)].

Authors:  F Lehmann-Grube; B Ibscher; E Bugislaus; M Kallay
Journal:  Med Microbiol Immunol       Date:  1979-08       Impact factor: 3.402

4.  Antibodies to the HFRS virus in the human population of European RSFSR as detected by radioimmunoassay.

Authors:  G V Rezapkin; Z V Shuikova; E A Tkachenko; A A Ivanova; R G Nurgaleeva; A G Stepanenko; N N Vereshchagin; A I Loginov; R N Bagan
Journal:  Arch Virol       Date:  1984       Impact factor: 2.574

5.  Detection of antibodies to varicella zoster virus by radioimmunoassay and enzyme immunoassay techniques.

Authors:  M G Friedman; H Haikin; S Leventon-Kriss; R Joffe; V Goldstein; I Sarov
Journal:  Med Microbiol Immunol       Date:  1978-11-17       Impact factor: 3.402
  5 in total

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