Literature DB >> 8755909

Conversion of NfsB, a minor Escherichia coli nitroreductase, to a flavin reductase similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri, by a single amino acid substitution.

S Zenno1, H Koike, M Tanokura, K Saigo.   

Abstract

NfsB is an oxygen-insensitive nitroreductase of Escherichia coli with significant amino acid sequence homology to the major flavin reductase (FRase I) of Vibrio fischeri. Here, we show that NfsB is convertible to an FRase I-like flavin reductase three times as active as the authentic FRase I by a single amino acid substitution in the least-conserved region.

Entities:  

Mesh：

Substances：

Year:  1996        PMID： 8755909      PMCID： PMC178248          DOI： 10.1128/jb.178.15.4731-4733.1996

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  Nucleotide sequence of Salmonella typhimurium nitroreductase gene.

Authors:  M Watanabe; M Ishidate; T Nohmi
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

2.  Characterization of the flavin reductase gene (fre) of Escherichia coli and construction of a plasmid for overproduction of the enzyme.

Authors:  G Spyrou; E Haggård-Ljungquist; M Krook; H Jörnvall; E Nilsson; P Reichard
Journal:  J Bacteriol       Date:  1991-06       Impact factor: 3.490

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Production of single-stranded plasmid DNA.

Authors:  J Vieira; J Messing
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

5.  A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.

Authors:  J Messing; J Vieira
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

6.  Identification of the gene encoding the major NAD(P)H-flavin oxidoreductase of the bioluminescent bacterium Vibrio fischeri ATCC 7744.

Authors:  S Zenno; K Saigo; H Kanoh; S Inouye
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

7.  NAD(P)H:flavin oxidoreductase of Escherichia coli. A ferric iron reductase participating in the generation of the free radical of ribonucleotide reductase.

Authors:  M Fontecave; R Eliasson; P Reichard
Journal:  J Biol Chem       Date:  1987-09-05       Impact factor: 5.157

8.  The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)--I. Purification and properties of a nitroreductase enzyme from Escherichia coli--a potential enzyme for antibody-directed enzyme prodrug therapy (ADEPT).

Authors:  G M Anlezark; R G Melton; R F Sherwood; B Coles; F Friedlos; R J Knox
Journal:  Biochem Pharmacol       Date:  1992-12-15       Impact factor: 5.858

9.  Physical characterisation of the Escherichia coli B gene encoding nitroreductase and its over-expression in Escherichia coli K12.

Authors:  N P Michael; J K Brehm; G M Anlezark; N P Minton
Journal:  FEMS Microbiol Lett       Date:  1994-12-01       Impact factor: 2.742

10.  Purification and analysis of a flavoprotein functional as NADH oxidase from Amphibacillus xylanus overexpressed in Escherichia coli.

Authors:  K Ohnishi; Y Niimura; K Yokoyama; M Hidaka; H Masaki; T Uchimura; H Suzuki; T Uozumi; M Kozaki; K Komagata; T Nishino
Journal:  J Biol Chem       Date:  1994-12-16       Impact factor: 5.157
View more
  14 in total

1.  Temperature dependence of the flexibility of thermophilic and mesophilic flavoenzymes of the nitroreductase fold.

Authors:  Eric D Merkley; William W Parson; Valerie Daggett
Journal:  Protein Eng Des Sel       Date:  2010-01-18       Impact factor: 1.650

2.  Conversion of NfsA, the major Escherichia coli nitroreductase, to a flavin reductase with an activity similar to that of Frp, a flavin reductase in Vibrio harveyi, by a single amino acid substitution.

Authors:  S Zenno; T Kobori; M Tanokura; K Saigo
Journal:  J Bacteriol       Date:  1998-01       Impact factor: 3.490

Review 3.  Biological degradation of 2,4,6-trinitrotoluene.

Authors:  A Esteve-Núñez; A Caballero; J L Ramos
Journal:  Microbiol Mol Biol Rev       Date:  2001-09       Impact factor: 11.056

4.  Chemoselective nitro group reduction and reductive dechlorination initiate degradation of 2-chloro-5-nitrophenol by Ralstonia eutropha JMP134.

Authors:  A Schenzle; H Lenke; J C Spain; H J Knackmuss
Journal:  Appl Environ Microbiol       Date:  1999-06       Impact factor: 4.792

5.  Biochemical characterization of NfsA, the Escherichia coli major nitroreductase exhibiting a high amino acid sequence homology to Frp, a Vibrio harveyi flavin oxidoreductase.

Authors:  S Zenno; H Koike; A N Kumar; R Jayaraman; M Tanokura; K Saigo
Journal:  J Bacteriol       Date:  1996-08       Impact factor: 3.490

6.  Mechanism-Informed Refinement Reveals Altered Substrate-Binding Mode for Catalytically Competent Nitroreductase.

Authors:  Warintra Pitsawong; Chad A Haynes; Ronald L Koder; David W Rodgers; Anne-Frances Miller
Journal:  Structure       Date:  2017-06-01       Impact factor: 5.006

7.  Oxygen-insensitive nitroreductases: analysis of the roles of nfsA and nfsB in development of resistance to 5-nitrofuran derivatives in Escherichia coli.

Authors:  J Whiteway; P Koziarz; J Veall; N Sandhu; P Kumar; B Hoecher; I B Lambert
Journal:  J Bacteriol       Date:  1998-11       Impact factor: 3.490

8.  Functions of flavin reductase and quinone reductase in 2,4,6-trichlorophenol degradation by Cupriavidus necator JMP134.

Authors:  Sara Mae Belchik; Luying Xun
Journal:  J Bacteriol       Date:  2007-12-28       Impact factor: 3.490

9.  Oxygen-insensitive nitroreductases NfsA and NfsB of Escherichia coli function under anaerobic conditions as lawsone-dependent Azo reductases.

Authors:  Jörg Rau; Andreas Stolz
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

10.  Fre Is the Major Flavin Reductase Supporting Bioluminescence from Vibrio harveyi Luciferase in Escherichia coli.

Authors:  Zachary T Campbell; Thomas O Baldwin
Journal:  J Biol Chem       Date:  2009-01-12       Impact factor: 5.157
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.