OBJECTIVE: To evaluate the possible advantages of reinseminating oocytes by use of intracytoplasmic sperm injection (ICSI). DESIGN: Clinical study. SETTING: In vitro fertilization unit with research facilities. PATIENTS: Fifty-seven couples who were part of our regular IVF program. INTERVENTIONS: Nonfertilized oocytes from IVF cycles with no or very low fertilization were microinjected with spermatozoa approximately 25 hours after oocyte pick-up. MAIN OUTCOME MEASURES: Fertilization and pregnancy rates. RESULTS: A mean fertilization rate of 46.5% was obtained when reinseminating the oocytes on day 2 using the ICSI procedure. Of 57 cycles with completely or almost completely failed fertilization, 29 patients received ET after reinsemination by ICSI. Two of these transfers resulted in pregnancies (6.9% per ET) and two healthy babies were born. CONCLUSION: Despite this relative success, considering both the extra work involved and the potential genetic risk, it is doubtful whether ICSI on day 2 should be recommended as a routine procedure. For training and research purposes, however, this approach can be of value.
OBJECTIVE: To evaluate the possible advantages of reinseminating oocytes by use of intracytoplasmic sperm injection (ICSI). DESIGN: Clinical study. SETTING: In vitro fertilization unit with research facilities. PATIENTS: Fifty-seven couples who were part of our regular IVF program. INTERVENTIONS: Nonfertilized oocytes from IVF cycles with no or very low fertilization were microinjected with spermatozoa approximately 25 hours after oocyte pick-up. MAIN OUTCOME MEASURES: Fertilization and pregnancy rates. RESULTS: A mean fertilization rate of 46.5% was obtained when reinseminating the oocytes on day 2 using the ICSI procedure. Of 57 cycles with completely or almost completely failed fertilization, 29 patients received ET after reinsemination by ICSI. Two of these transfers resulted in pregnancies (6.9% per ET) and two healthy babies were born. CONCLUSION: Despite this relative success, considering both the extra work involved and the potential genetic risk, it is doubtful whether ICSI on day 2 should be recommended as a routine procedure. For training and research purposes, however, this approach can be of value.
Authors: Tugce Pehlivan; Carmen Rubio; Amparo Ruiz; Jose Navarro; José Remohí; Antonio Pellicer; Carlos Simón Journal: J Assist Reprod Genet Date: 2004-02 Impact factor: 3.412