Literature DB >> 8748299

Comparison of two leukocyte extraction methods for cytomegalovirus antigenemia assay.

A García1, J Niubò, M A Benítez, M Viqueira, J L Pérez.   

Abstract

We carried out a prospective, parallel, and blind study on 113 blood samples from immunocompromised patients in order to compare two leukocyte extraction methods (6% dextran sedimentation and Polymorphprep separation) for cytomegalovirus (CMV) antigenemia assay. CMV was detected in 38 samples by antigenemia assay (34 by dextran sedimentation and 35 by Polymorphprep separation). No differences either in the number of positive specimens (P = 1) or in the mean CMV-positive cell counts (P = 0.41) were observed between the two leukocyte extraction methods. In conclusion, the two methods performed equally well for this assay.

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Year:  1996        PMID： 8748299      PMCID： PMC228756          DOI： 10.1128/jcm.34.1.182-184.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

Review 1.  Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction.

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Journal:  Transplantation       Date:  1992-08       Impact factor: 4.939

2.  Human cytomegalovirus infection of the major leukocyte subpopulations and evidence for initial viral replication in polymorphonuclear leukocytes from viremic patients.

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Journal:  J Infect Dis       Date:  1992-12       Impact factor: 5.226

3.  Evaluation of CMV-vue antigenemia assay for rapid detection of cytomegalovirus in mixed-leukocyte blood fractions.

Authors:  A Erice; M A Holm; M V Sanjuan; D L Dunn; P C Gill; H H Balfour
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948

4.  Comparison of three commercially available monoclonal antibodies directed against pp65 antigen for cytomegalovirus antigenemia assay.

Authors:  J L Pérez; J Niubò; C Ardanuy; A Carvajal; A García; J Salvà; J T Martínez-Lacasa; J M Escribà; R Martín
Journal:  Diagn Microbiol Infect Dis       Date:  1995-01       Impact factor: 2.803

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Journal:  Enferm Infecc Microbiol Clin       Date:  1994-05       Impact factor: 1.731

6.  Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hybridization.

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Journal:  J Infect Dis       Date:  1990-01       Impact factor: 5.226

7.  Cytomegalovirus excretion as a predictor of cytomegalovirus disease after marrow transplantation: importance of cytomegalovirus viremia.

Authors:  J D Meyers; P Ljungman; L D Fisher
Journal:  J Infect Dis       Date:  1990-08       Impact factor: 5.226

8.  Cytomegalovirus (CMV) antigenemia assay is more sensitive than shell vial cultures for rapid detection of CMV in polymorphonuclear blood leukocytes.

Authors:  A Erice; M A Holm; P C Gill; S Henry; C L Dirksen; D L Dunn; R P Hillam; H H Balfour
Journal:  J Clin Microbiol       Date:  1992-11       Impact factor: 5.948

9.  Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells.

Authors:  J Taylor-Wiedeman; J G Sissons; L K Borysiewicz; J H Sinclair
Journal:  J Gen Virol       Date:  1991-09       Impact factor: 3.891

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Authors:  J T Martínez Lacasa; D Podzamczer; G Rufí; F Bolao; J L Pérez; F Gudiol
Journal:  Med Clin (Barc)       Date:  1993-01-16       Impact factor: 1.725
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  8 in total

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Authors:  S K Ho; C Y Lo; I K Cheng; T M Chan
Journal:  J Clin Microbiol       Date:  1998-03       Impact factor: 5.948

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Authors:  Tuan M Nguyen; Dipti Ravindra; Brian Kwong; Sana Waheed; Ryan Ferguson; Nicole Tarlton; Victoria Wu; Christopher S Sequeira; Martina Bremer; Tzvia Abramson
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5.  Determination of human cytomegalovirus pp65 antigenemia among renal transplant patients.

Authors:  S Moses; J Malathi; N R Singha; R Bagyalakshmi; H N Madhavan
Journal:  Indian J Nephrol       Date:  2012-09

6.  Neutrophil isolation protocol.

Authors:  Hana Oh; Brian Siano; Scott Diamond
Journal:  J Vis Exp       Date:  2008-07-23       Impact factor: 1.355

7.  Canine GM2-Gangliosidosis Sandhoff Disease Associated with a 3-Base Pair Deletion in the HEXB Gene.

Authors:  P Wang; P S Henthorn; E Galban; G Lin; T Takedai; M Casal
Journal:  J Vet Intern Med       Date:  2017-11-06       Impact factor: 3.333

8.  An explorative study identifies miRNA signatures for the diagnosis of non-celiac wheat sensitivity.

Authors:  Emanuela Clemente; Konstantinos Efthymakis; Erminia Carletti; Vanessa Capone; Samantha Sperduti; Giuseppina Bologna; Marco Marchisio; Marta Di Nicola; Matteo Neri; Michele Sallese
Journal:  PLoS One       Date:  2019-12-13       Impact factor: 3.240
  8 in total

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