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Literature DB >> 8747480

Quantitative analysis of pulmonary surfactant proteins B and C.

Abstract

A procedure for the quantification of surfactant protein B (SP-B) and surfactant protein C (SP-C) in surfactant preparations is described. After butanol extraction of surfactant, the resulting extract is separated by Sephadex LH-60 HPLC, using an elution fluid which consists of 30% dichloromethane, 65% methanol, and 5% 100 mM HCL. SP-B and SP-C are detected by absorbance at 228 nm and quantified using standard stock solutions. Run length is approximately 70 min. Detection limits are 1 microgram SP-B and 4 micrograms SP-C.

Entities: Chemical Gene

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Year: 1995 PMID： 8747480 DOI： 10.1006/abio.1995.0012

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

11 in total

1. Hydrophobic surfactant proteins induce a phosphatidylethanolamine to form cubic phases.

Authors: Mariya Chavarha; Hamed Khoojinian; Leonard E Schulwitz; Samares C Biswas; Shankar B Rananavare; Stephen B Hall
Journal: Biophys J Date: 2010-04-21 Impact factor: 4.033

2. Hydrophobic surfactant proteins strongly induce negative curvature.

Authors: Mariya Chavarha; Ryan W Loney; Shankar B Rananavare; Stephen B Hall
Journal: Biophys J Date: 2015-07-07 Impact factor: 4.033

3. Neutral lipids induce critical behavior in interfacial monolayers of pulmonary surfactant.

Authors: B M Discher; K M Maloney; D W Grainger; C A Sousa; S B Hall
Journal: Biochemistry Date: 1999-01-05 Impact factor: 3.162

4. Establishment of LC-MS methods for the analysis of palmitoylated surfactant proteins.

Authors: Takeshi Harayama; Hideo Shindou; Yoshihiro Kita; Eiji Otsubo; Kazushige Ikeda; Shoichi Chida; Timothy E Weaver; Takao Shimizu
Journal: J Lipid Res Date: 2015-05-28 Impact factor: 5.922

5. Lucinactant attenuates pulmonary inflammatory response, preserves lung structure, and improves physiologic outcomes in a preterm lamb model of RDS.

Authors: Marla R Wolfson; Jichuan Wu; Terrence L Hubert; Timothy J Gregory; Jan Mazela; Thomas H Shaffer
Journal: Pediatr Res Date: 2012-07-20 Impact factor: 3.756

6. Reverse-phase HPLC of the hydrophobic pulmonary surfactant proteins: detection of a surfactant protein C isoform containing Nepsilon-palmitoyl-lysine.

Authors: M Gustafsson; T Curstedt; H Jörnvall; J Johansson
Journal: Biochem J Date: 1997-09-15 Impact factor: 3.857

Quantitative analysis of pulmonary surfactant proteins B and C.

1. Hydrophobic surfactant proteins induce a phosphatidylethanolamine to form cubic phases.

2. Hydrophobic surfactant proteins strongly induce negative curvature.

3. Neutral lipids induce critical behavior in interfacial monolayers of pulmonary surfactant.

4. Establishment of LC-MS methods for the analysis of palmitoylated surfactant proteins.

5. Lucinactant attenuates pulmonary inflammatory response, preserves lung structure, and improves physiologic outcomes in a preterm lamb model of RDS.

6. Reverse-phase HPLC of the hydrophobic pulmonary surfactant proteins: detection of a surfactant protein C isoform containing Nepsilon-palmitoyl-lysine.

7. Surface properties, morphology and protein composition of pulmonary surfactant subtypes.

Review 8. Recent Developments in mRNA-Based Protein Supplementation Therapy to Target Lung Diseases.

9. An anionic phospholipid enables the hydrophobic surfactant proteins to alter spontaneous curvature.

10. SP-B and SP-C alter diffusion in bilayers of pulmonary surfactant.