Literature DB >> 8743586

Characterization of a B. subtilis minor isoleucine tRNA deduced from tDNA having a methionine anticodon CAT.

J Matsugi1, K Murao, H Ishikura.   

Abstract

Bacillus subtilis, which belongs to Gram-positive eubacteria, has been predicted to have a minor isoleucine tRNA transcribed from the gene possessing the CAT anticodon, which corresponds to methionine. We isolated this tRNA and determined its sequence including modified nucleotides. Modified nucleotide analyses using TLC, UV, and FAB mass spectroscopy revealed that the first letter of the anticodon is modified to lysidine [4-amino-2-(N6-lysino)-1-beta-d-ribofuranosyl pyrimidine]. As a result, this tRNA agrees with the minor one predicted from the DNA sequence and is thought to decode the isoleucine codon AUA.

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Year:  1996        PMID： 8743586     DOI： 10.1093/oxfordjournals.jbchem.a021312

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  10 in total

1.  Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product.

Authors:  G Plunkett; D J Rose; T J Durfee; F R Blattner
Journal:  J Bacteriol       Date:  1999-03       Impact factor: 3.490

2.  tRNomics: analysis of tRNA genes from 50 genomes of Eukarya, Archaea, and Bacteria reveals anticodon-sparing strategies and domain-specific features.

Authors:  Christian Marck; Henri Grosjean
Journal:  RNA       Date:  2002-10       Impact factor: 4.942

3.  Thermus thermophilus bacteriophage phiYS40 genome and proteomic characterization of virions.

Authors:  Tatyana Naryshkina; Jing Liu; Laurence Florens; Selene K Swanson; Andrey R Pavlov; Nadejda V Pavlova; Ross Inman; Leonid Minakhin; Sergei A Kozyavkin; Michael Washburn; Arcady Mushegian; Konstantin Severinov
Journal:  J Mol Biol       Date:  2006-09-06       Impact factor: 5.469

4.  Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase.

Authors:  Kotaro Nakanishi; Luc Bonnefond; Satoshi Kimura; Tsutomu Suzuki; Ryuichiro Ishitani; Osamu Nureki
Journal:  Nature       Date:  2009-10-22       Impact factor: 49.962

5.  Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.

Authors:  D R Smith; L A Doucette-Stamm; C Deloughery; H Lee; J Dubois; T Aldredge; R Bashirzadeh; D Blakely; R Cook; K Gilbert; D Harrison; L Hoang; P Keagle; W Lumm; B Pothier; D Qiu; R Spadafora; R Vicaire; Y Wang; J Wierzbowski; R Gibson; N Jiwani; A Caruso; D Bush; J N Reeve
Journal:  J Bacteriol       Date:  1997-11       Impact factor: 3.490

6.  Structural basis for lysidine formation by ATP pyrophosphatase accompanied by a lysine-specific loop and a tRNA-recognition domain.

Authors:  Kotaro Nakanishi; Shuya Fukai; Yoshiho Ikeuchi; Akiko Soma; Yasuhiko Sekine; Tsutomu Suzuki; Osamu Nureki
Journal:  Proc Natl Acad Sci U S A       Date:  2005-05-13       Impact factor: 11.205

7.  The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase.

Authors:  Scott P Salowe; Judyann Wiltsie; Julio C Hawkins; Lisa M Sonatore
Journal:  J Biol Chem       Date:  2009-02-19       Impact factor: 5.157

8.  Complete genome of the broad-host-range Erwinia amylovora phage phiEa21-4 and its relationship to Salmonella phage felix O1.

Authors:  Susan M Lehman; Andrew M Kropinski; Alan J Castle; Antonet M Svircev
Journal:  Appl Environ Microbiol       Date:  2009-01-30       Impact factor: 4.792

9.  Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis.

Authors:  Brian P Anton; Susan P Russell; Jason Vertrees; Simon Kasif; Elisabeth A Raleigh; Patrick A Limbach; Richard J Roberts
Journal:  Nucleic Acids Res       Date:  2010-05-14       Impact factor: 16.971

10.  Life without tRNAIle-lysidine synthetase: translation of the isoleucine codon AUA in Bacillus subtilis lacking the canonical tRNA2Ile.

Authors:  Caroline Köhrer; Debabrata Mandal; Kirk W Gaston; Henri Grosjean; Patrick A Limbach; Uttam L Rajbhandary
Journal:  Nucleic Acids Res       Date:  2013-11-04       Impact factor: 16.971
  10 in total

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