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Literature DB >> 8743048

Sterol specificity of the Saccharomyces cerevisiae ERG6 gene product expressed in Escherichia coli.

M Venkatramesh¹, D A Guo, J G Harman, W D Nes.

Abstract

The ERG6 gene from Saccharomyces cerevisiae has been functionally expressed in Escherichia coli, for the first time, yielding a protein that catalyzes the bisubstrate transfer reaction whereby the reactive methyl group from (S)-adenosyl-L-methionine is transferred stereoselectively to C-24 of the sterol side chain. The structural requirements of sterol in binding and catalysis were similar to the native protein from S. cerevisiae. Inhibition of biomethylation was observed with fecosterol and ergosterol which suggests that ergosterol may function in wild-type yeast as feedback regulator of sterol biosynthesis.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1996 PMID： 8743048 DOI： 10.1007/bf02522922

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

18 in total

1. Mechanism and inhibition of delta 24-sterol methyltransferase from Candida albicans and Candida tropicalis.

Authors: M A Ator; S J Schmidt; J L Adams; R E Dolle
Journal: Biochemistry Date: 1989-12-12 Impact factor: 3.162

2. A simplified method for the quantitative assay of small amounts of protein in biologic material.

Authors: G R Schacterle; R L Pollack
Journal: Anal Biochem Date: 1973-02 Impact factor: 3.365

3. Investigation of an S-adenosylmethionine: delta 24-sterol methyltransferase in ergosterol biosynthesis in yeast. Specificity of sterol substrates and inhibitors.

Authors: J T Moore; J L Gaylor
Journal: J Biol Chem Date: 1970-09-25 Impact factor: 5.157

Review 4. Inhibitors of sterol biosynthesis and their applications.

Authors: E I Mercer
Journal: Prog Lipid Res Date: 1993 Impact factor: 16.195

5. Azasterol inhibition of delta 24-sterol methyltransferase in Saccharomyces cerevisiae.

Authors: A C Oehlschlager; R H Angus; A M Pierce; H D Pierce; R Srinivasan
Journal: Biochemistry Date: 1984-07-31 Impact factor: 3.162

6. Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae.

Authors: A A Welihinda; A D Beavis; R J Trumbly
Journal: Biochim Biophys Acta Date: 1994-07-13

7. Sterol methylation in Saccharomyces cerevisiae.

Authors: M T McCammon; M A Hartmann; C D Bottema; L W Parks
Journal: J Bacteriol Date: 1984-02 Impact factor: 3.490

8. Structural requirements for transformation of substrates by the S-adenosyl-L-methionine:delta 24(25)-sterol methyltransferase. Inhibition by analogs of the transition state coordinate.

Authors: G G Janssen; W D Nes
Journal: J Biol Chem Date: 1992-12-25 Impact factor: 5.157

9. Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae.

Authors: M Venkatramesh; D A Guo; Z Jia; W D Nes
Journal: Biochim Biophys Acta Date: 1996-02-16

Review 6. Sterol biosynthesis inhibitors: potential for transition state analogs and mechanism-based inactivators targeted at sterol methyltransferase.

Authors: Zhihong Song; W David Nes
Journal: Lipids Date: 2007-02-14 Impact factor: 1.880

Review 7. Steroidal triterpenes: design of substrate-based inhibitors of ergosterol and sitosterol synthesis.

Authors: Jialin Liu; William David Nes
Journal: Molecules Date: 2009-11-18 Impact factor: 4.411

7 in total

Sterol specificity of the Saccharomyces cerevisiae ERG6 gene product expressed in Escherichia coli.

1. Mechanism and inhibition of delta 24-sterol methyltransferase from Candida albicans and Candida tropicalis.

2. A simplified method for the quantitative assay of small amounts of protein in biologic material.

3. Investigation of an S-adenosylmethionine: delta 24-sterol methyltransferase in ergosterol biosynthesis in yeast. Specificity of sterol substrates and inhibitors.

Review 4. Inhibitors of sterol biosynthesis and their applications.

5. Azasterol inhibition of delta 24-sterol methyltransferase in Saccharomyces cerevisiae.

6. Mutations in LIS1 (ERG6) gene confer increased sodium and lithium uptake in Saccharomyces cerevisiae.

7. Sterol methylation in Saccharomyces cerevisiae.

8. Structural requirements for transformation of substrates by the S-adenosyl-L-methionine:delta 24(25)-sterol methyltransferase. Inhibition by analogs of the transition state coordinate.

9. Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae.

10. Studies on transformation of Escherichia coli with plasmids.

1. Sterol metabolism.

Review 2. Mechanism-based enzyme inactivators of phytosterol biosynthesis.

3. Plant sterol-C24-methyl transferases: different profiles of tobacco transformed with SMT1 or SMT2.

Review 4. Advances in steroidal saponins biosynthesis.

5. Enhanced membrane fusion in sterol-enriched vacuoles bypasses the Vrp1p requirement.

Review 6. Sterol biosynthesis inhibitors: potential for transition state analogs and mechanism-based inactivators targeted at sterol methyltransferase.

Review 7. Steroidal triterpenes: design of substrate-based inhibitors of ergosterol and sitosterol synthesis.