Literature DB >> 8742746

CTF determination of images of ice-embedded single particles using a graphics interface.

Z H Zhou1, S Hardt, B Wang, M B Sherman, J Jakana, W Chiu.   

Abstract

We implement a graphical user interface in an X-window/UNIX environment to compute and display the incoherently averaged Fourier transforms of electron images of single particles embedded in ice and the simulated contrast transfer function with or without envelope functions. This interface provides an easy and efficient operation for the determination of defocus value and the evaluation of the extent of Fourier amplitude falloff. This computational procedure is crucial for prescreening image data and performing image correction of contrast transfer function in high-resolution three-dimensional reconstruction of single particles.

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Year:  1996        PMID： 8742746     DOI： 10.1006/jsbi.1996.0033

Source DB:  PubMed          Journal:  J Struct Biol        ISSN： 1047-8477            Impact factor:   2.867


  32 in total

1.  Roles of triplex and scaffolding proteins in herpes simplex virus type 1 capsid formation suggested by structures of recombinant particles.

Authors:  A Saad; Z H Zhou; J Jakana; W Chiu; F J Rixon
Journal:  J Virol       Date:  1999-08       Impact factor: 5.103

2.  Three-dimensional structure of low density lipoproteins by electron cryomicroscopy.

Authors:  E V Orlova; M B Sherman; W Chiu; H Mowri; L C Smith; A M Gotto
Journal:  Proc Natl Acad Sci U S A       Date:  1999-07-20       Impact factor: 11.205

3.  Solution x-ray scattering-based estimation of electron cryomicroscopy imaging parameters for reconstruction of virus particles.

Authors:  P A Thuman-Commike; H Tsuruta; B Greene; P E Prevelige; J King; W Chiu
Journal:  Biophys J       Date:  1999-04       Impact factor: 4.033

4.  The remarkable structural and functional organization of the eukaryotic pyruvate dehydrogenase complexes.

Authors:  Z H Zhou; D B McCarthy; C M O'Connor; L J Reed; J K Stoops
Journal:  Proc Natl Acad Sci U S A       Date:  2001-12-18       Impact factor: 11.205

5.  Mechanism of scaffolding-directed virus assembly suggested by comparison of scaffolding-containing and scaffolding-lacking P22 procapsids.

Authors:  P A Thuman-Commike; B Greene; J A Malinski; M Burbea; A McGough; W Chiu; P E Prevelige
Journal:  Biophys J       Date:  1999-06       Impact factor: 4.033

6.  Using cryo-EM to measure the dipole potential of a lipid membrane.

Authors:  Liguo Wang; Pulkit S Bose; Fred J Sigworth
Journal:  Proc Natl Acad Sci U S A       Date:  2006-11-20       Impact factor: 11.205

Review 7.  3D electron microscopy of biological nanomachines: principles and applications.

Authors:  C O S Sorzano; S Jonic; M Cottevieille; E Larquet; N Boisset; S Marco
Journal:  Eur Biophys J       Date:  2007-07-05       Impact factor: 1.733

8.  Identification of the sites of interaction between the scaffold and outer shell in herpes simplex virus-1 capsids by difference electron imaging.

Authors:  Z H Zhou; S J Macnab; J Jakana; L R Scott; W Chiu; F J Rixon
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-17       Impact factor: 11.205

9.  Refinement of herpesvirus B-capsid structure on parallel supercomputers.

Authors:  Z H Zhou; W Chiu; K Haskell; H Spears; J Jakana; F J Rixon; L R Scott
Journal:  Biophys J       Date:  1998-01       Impact factor: 4.033

10.  Role of the scaffolding protein in P22 procapsid size determination suggested by T = 4 and T = 7 procapsid structures.

Authors:  P A Thuman-Commike; B Greene; J A Malinski; J King; W Chiu
Journal:  Biophys J       Date:  1998-01       Impact factor: 4.033
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