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Literature DB >> 873901

Specific cytosol-binding protein for 1,25-dihydroxyvitamin D3 in rat intestine.

B E Kream, S Yamada, H K Schnoes, H F DeLuca.

Abstract

Cytosol prepared from intestinal homogenates of vitamin D3-deficient rats demonstrate a 3.2 S protein having high affinity and low capacity for 1,25-dihydroxyvitamin D3. Appearance of the protein is dependent upon the presence of 0.3 M potassium chloride and dithiothreitol in the homogenization buffer. A 6 S protein having greater affinity for 25-hydroxyvitamin D3 than 1,25-dihydroxyvitamin D3 is observed using all conditions and is increased by the addition of rat serum to cytosol. The 3.2 S protein is neither a serum contaminant nor a component of the 6 S protein and is probably not a consituent of tissues which which are not responsive to vitamin D3.

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Year: 1977 PMID： 873901

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

13 in total

1. Sertoli cells in the testis and epithelium of the ductuli efferentes are targets for 3H 1,25 (OH)2 vitamin D3. An autoradiographic study.

Authors: W E Stumpf; M Sar; K Chen; J Morin; H F DeLuca
Journal: Cell Tissue Res Date: 1987-02 Impact factor: 5.249

10. Identification of the porcine intestinal 1,25-dihydroxyvitamin D3 receptor on sodium dodecyl sulfate/polyacrylamide gels by renaturation and immunoblotting.

Authors: M C Dame; E A Pierce; H F DeLuca
Journal: Proc Natl Acad Sci U S A Date: 1985-12 Impact factor: 11.205

Specific cytosol-binding protein for 1,25-dihydroxyvitamin D3 in rat intestine.

1. Sertoli cells in the testis and epithelium of the ductuli efferentes are targets for 3H 1,25 (OH)2 vitamin D3. An autoradiographic study.

2. The avian vitamin D receptors: primary structures and their origins.

3. Effect of ligand binding on the conformation of human plasma vitamin D binding protein (group-specific component).

4. A monolog on analogs: in vitro effects of vitamin D metabolites and consideration of the mineralization question.

5. Evidence for 24,25-dihydroxycholecalciferol receptors in long bones of newborn rats.

6. Characterization of a receptor-like protein for 1,25-dihydroxyvitamin D3 in rat skin.

7. A high-affinity cytosol binding protein for 1 alpha,25-dihydroxycholecalciferol in the uterus of Japanese quail.

8. Oxygen-dependent 1,25-dihydroxycholecalciferol-induced calcium ion transport in rat intestine.

9. Evidence for in vivo upregulation of the intestinal vitamin D receptor during dietary calcium restriction in the rat.

10. Identification of the porcine intestinal 1,25-dihydroxyvitamin D3 receptor on sodium dodecyl sulfate/polyacrylamide gels by renaturation and immunoblotting.