| Literature DB >> 8737666 |
C Leloup1, M Arluison, N Kassis, N Lepetit, N Cartier, P Ferré, L Pénicaud.
Abstract
Whether or not glucose utilization in the brain is insulin-dependent is still a controversial issue. We looked for the presence of the insulin-sensitive glucose transporter (GLUT4) in rat brain and obtained the following results: (1) poly(A) RNAs from the hypothalamus and anterior medulla oblongata hybridize with a cDNA probe for GLUT4; (2) reverse transcription-polymerase chain reaction (RT-PCR) on RNA from various brain nuclei detects GLUT4 transcripts; (3) immunocytochemistry, using a polyclonal antibody to GLUT4; reveals a specific immunostaining pattern, whereas both electronic microscopy and double immunofluorescence staining, using a neurofilament protein marker, indicate a neuronal localization. These results are discussed in terms of a putative neuromodulator role of insulin, via glucose utilization, in brain areas involved in the regulation of fuel metabolism.Entities:
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Year: 1996 PMID： 8737666 DOI： 10.1016/0169-328x(95)00306-d
Source DB: PubMed Journal: Brain Res Mol Brain Res ISSN： 0169-328X