Literature DB >> 8735523

Detection of Fasciola hepatica antigen in cattle faeces by a monoclonal antibody-based sandwich immunoassay.

B E Duménigo1, A M Espino, C M Finlay.   

Abstract

A monoclonal antibody-based sandwich immunoassay (mAb sandwich ELISA) was developed for the detection of Fasciola hepatica antigen in the faeces of cattle. The assay was applied to samples from 100 cattle infected with F hepatica, 56 animals with parasitologically proven infections of other parasites and 100 uninfected animals. F hepatica antigen was detected in all the faecal samples from animals with fasciolosis, but none of the samples from the uninfected animals or from those with other parasitic infections had significant levels of F hepatica antigens. The results indicate that the mAb sandwich ELISA is a rapid, simple and useful method for the diagnosis of active F hepatica infection in cattle.

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Year:  1996        PMID： 8735523     DOI： 10.1016/s0034-5288(96)90055-7

Source DB:  PubMed          Journal:  Res Vet Sci        ISSN： 0034-5288            Impact factor:   2.534


  7 in total

1.  Diagnostic imaging in sheep hepatic fascioliasis: ultrasound, computer tomography and magnetic resonance findings.

Authors:  M Gonzalo-Orden; L Millán; M Alvarez; S Sánchez-Campos; R Jiménez; J González-Gallego; M J Tuñón
Journal:  Parasitol Res       Date:  2003-04-17       Impact factor: 2.289

2.  Evaluation of a polyclonal antibody based sandwich ELISA for the detection of faecal antigens in Schistosoma spindale infection in bovines.

Authors:  G S Sreenivasa Murthy; Placid E D'Souza; K Shrikrishna Isloor
Journal:  J Parasit Dis       Date:  2012-06-24

3.  Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak.

Authors:  A M Espino; A Díaz; A Pérez; C M Finlay
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

4.  Prevalence of natural ovine fasciolosis shown by demonstrating the presence of serum circulating antigens.

Authors:  A Paz-Silva; R Sánchez-Andrade; J L Suárez; J Pedreira; M Arias; C López; R Panadero; P Díaz; P Díez-Baños; P Morrondo
Journal:  Parasitol Res       Date:  2003-09-09       Impact factor: 2.289

5.  Immunodetection of Fasciola gigantica circulating antigen in sera of infected individuals for laboratory diagnosis of human fascioliasis.

Authors:  Abdelfattah M Attallah; Faisal A Bughdadi; Atef M El-Shazly; Hisham Ismail
Journal:  Clin Vaccine Immunol       Date:  2013-08-14

6.  Field evaluation of a coproantigen detection test for fascioliasis diagnosis and surveillance in human hyperendemic areas of Andean countries.

Authors:  María Adela Valero; María Victoria Periago; Ignacio Pérez-Crespo; René Angles; Fidel Villegas; Carlos Aguirre; Wilma Strauss; José R Espinoza; Patricia Herrera; Angelica Terashima; Hugo Tamayo; Dirk Engels; Albis Francesco Gabrielli; Santiago Mas-Coma
Journal:  PLoS Negl Trop Dis       Date:  2012-09-13

7.  Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool.

Authors:  Zeinab A Demerdash; Tarek M Diab; Ibrahim R Aly; Salwa H Mohamed; Faten S Mahmoud; Mona K Zoheiry; Wafaa A Mansour; Mohy E Attia; Azza E El-Bassiouny
Journal:  Parasit Vectors       Date:  2011-09-15       Impact factor: 3.876
  7 in total

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