Literature DB >> 8735099

Detection of enteroviruses from clinical specimens by spin amplification shell vial culture and monoclonal antibody assay.

S L Klespies1, D E Cebula, C L Kelley, D Galehouse, C C Maurer.   

Abstract

Conventional tube cell culture was compared with a 72-h, spin-amplified shell vial indirect immunofluorescence assay for the detection of enterovirus from clinical specimens. The sensitivity for the shell vial assay after resolution of discrepant results were 93 and 100%, respectively. The shell vial assay detected 93% of the positive cultures within 72 h of incubation while conventional tube culture detected only 51% of the positive cultures within the same time interval. The data suggest that a spin-amplified shell vial indirect immunofluorescence assay may be useful for the detection of enterovirus from clinical specimens.

Mesh：

Substances：

Year:  1996        PMID： 8735099      PMCID： PMC229043          DOI： 10.1128/jcm.34.6.1465-1467.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Spectrum of monoclonal antibodies to coxsackievirus B-3 includes type- and group-specific antibodies.

Authors:  S Yagi; D Schnurr; J Lin
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Identification of enteroviruses by indirect immunofluorescence using monoclonal antibodies.

Authors:  D Bastis; S Simonet; M A Patterson; S Neill
Journal:  Clin Diagn Virol       Date:  1995-01

3.  Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation.

Authors:  C A Gleaves; D J Wilson; A D Wold; T F Smith
Journal:  J Clin Microbiol       Date:  1985-01       Impact factor: 5.948

4.  Clinical and research application of an enterovirus group-reactive monoclonal antibody.

Authors:  G E Yousef; G F Mann; I N Brown; J F Mowbray
Journal:  Intervirology       Date:  1987       Impact factor: 1.763

5.  Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody, 5-D8/1, in comparison with neutralization and PCR for rapid identification of enteroviruses in cell culture.

Authors:  A Trabelsi; F Grattard; M Nejmeddine; M Aouni; T Bourlet; B Pozzetto
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

6.  Use of murine monoclonal antibodies for laboratory diagnosis of varicella-zoster virus infection.

Authors:  C A Gleaves; C F Lee; C I Bustamante; J D Meyers
Journal:  J Clin Microbiol       Date:  1988-09       Impact factor: 5.948

7.  The isolation of nonpolio enteroviruses from serum.

Authors:  S L Prather; J A Jenista; M A Menegus
Journal:  Diagn Microbiol Infect Dis       Date:  1984-09       Impact factor: 2.803

8.  Use of immunofluorescence to identify measles virus infections.

Authors:  L L Minnich; F Goodenough; C G Ray
Journal:  J Clin Microbiol       Date:  1991-06       Impact factor: 5.948

9.  Comparison of cell cultures for rapid isolation of enteroviruses.

Authors:  T Chonmaitree; C Ford; C Sanders; H L Lucia
Journal:  J Clin Microbiol       Date:  1988-12       Impact factor: 5.948

10.  The clinical relevance of 'CSF viral culture'. A two-year experience with aseptic meningitis in Rochester, NY.

Authors:  T Chonmaitree; M A Menegus; K R Powell
Journal:  JAMA       Date:  1982-04-02       Impact factor: 56.272
View more
  12 in total

1.  Molecular detection and identification of an enterovirus during an outbreak of aseptic meningitis.

Authors:  N Siafakas; A Georgopoulou; P Markoulatos; N Spyrou; G Stanway
Journal:  J Clin Lab Anal       Date:  2001       Impact factor: 2.352

2.  Detection of precytopathic effect of enteroviruses in clinical specimens by centrifugation-enhanced antigen detection.

Authors:  S M Lipson; K David; F Shaikh; L Qian
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

3.  Engineered BGMK cells for sensitive and rapid detection of enteroviruses.

Authors:  Yung T Huang; Paul Yam; Huimin Yan; Yan Sun
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

Review 4.  Role of cell culture for virus detection in the age of technology.

Authors:  Diane S Leland; Christine C Ginocchio
Journal:  Clin Microbiol Rev       Date:  2007-01       Impact factor: 26.132

5.  Rapid and highly sensitive coxsackievirus a indirect immunofluorescence assay typing kit for enterovirus serotyping.

Authors:  Tsuey-Li Lin; Yi-Syue Li; Chiao-Wei Huang; Chiu-Chu Hsu; Ho-Sheng Wu; Tsan-Chang Tseng; Chen-Fu Yang
Journal:  J Clin Microbiol       Date:  2007-11-21       Impact factor: 5.948

Review 6.  Molecular typing of enteroviruses: current status and future requirements. The European Union Concerted Action on Virus Meningitis and Encephalitis.

Authors:  P Muir; U Kämmerer; K Korn; M N Mulders; T Pöyry; B Weissbrich; R Kandolf; G M Cleator; A M van Loon
Journal:  Clin Microbiol Rev       Date:  1998-01       Impact factor: 26.132

7.  Variability in molecular typing of Coxsackie A viruses by RFLP analysis and sequencing.

Authors:  Nikolaos Siafakas; Panayotis Markoulatos; Glyn Stanway
Journal:  J Clin Lab Anal       Date:  2002       Impact factor: 2.352

Review 8.  Detection of respiratory viruses by molecular methods.

Authors:  James B Mahony
Journal:  Clin Microbiol Rev       Date:  2008-10       Impact factor: 26.132

9.  Monoclonal antibodies to VP1 recognize a broad range of enteroviruses.

Authors:  Lynn Yihong Miao; Christina Pierce; Jennifer Gray-Johnson; Jill DeLotell; Carl Shaw; Nate Chapman; Elaine Yeh; David Schnurr; Yung T Huang
Journal:  J Clin Microbiol       Date:  2009-08-26       Impact factor: 5.948

10.  Bilirubin: An Endogenous Molecule with Antiviral Activity in vitro.

Authors:  Rosaria Santangelo; Cesare Mancuso; Simona Marchetti; Enrico Di Stasio; Giovambattista Pani; Giovanni Fadda
Journal:  Front Pharmacol       Date:  2012-03-09       Impact factor: 5.810
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.