Literature DB >> 8733229

Purification of recombinant Chlamydia trachomatis histone H1-like protein Hc2, and comparative functional analysis of Hc2 and Hc1.

L B Pedersen1, S Birkelund, G Christiansen.   

Abstract

The metabolically inactive developmental form of Chlamydia trachomatis, the elementary body, contains two very basic DNA-binding proteins with homology to eukaryotic histone H1. One of these, Hc1, is relatively well characterized and induces DNA condensation in vitro, whereas the other, Hc2, is functionally virtually uncharacterized. In this study we describe the purification of Hc2, and a detailed comparative functional analysis of Hc2 and Hc1 is presented. By gel shift assays and electron microscopy, marked differences in the nucleic acid-binding properties of Hc2 and Hc1 were observed. Furthermore, Hc2 was found to strongly inhibit translation and transcription in vitro. Our results imply that DNA condensation is not the only function of Hc2.

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Year:  1996        PMID： 8733229     DOI： 10.1111/j.1365-2958.1996.tb02618.x

Source DB:  PubMed          Journal:  Mol Microbiol        ISSN： 0950-382X            Impact factor:   3.501


  11 in total

1.  Sigma28 RNA polymerase regulates hctB, a late developmental gene in Chlamydia.

Authors:  Hilda Hiu Yin Yu; Ming Tan
Journal:  Mol Microbiol       Date:  2003-10       Impact factor: 3.501

2.  Regulation of the Chlamydia trachomatis histone H1-like protein Hc2 is IspE dependent and IhtA independent.

Authors:  Nicole A Grieshaber; Janet Burgess Sager; Cheryl A Dooley; Stanley F Hayes; Ted Hackstadt
Journal:  J Bacteriol       Date:  2006-07       Impact factor: 3.490

Review 3.  Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen.

Authors:  Robert J Bastidas; Raphael H Valdivia
Journal:  Microbiol Mol Biol Rev       Date:  2016-03-30       Impact factor: 11.056

4.  Characterization of a Chlamydia psittaci DNA binding protein (EUO) synthesized during the early and middle phases of the developmental cycle.

Authors:  L Zhang; A L Douglas; T P Hatch
Journal:  Infect Immun       Date:  1998-03       Impact factor: 3.441

5.  Drosophila melanogaster S2 cells: a model system to study Chlamydia interaction with host cells.

Authors:  C Elwell; J N Engel
Journal:  Cell Microbiol       Date:  2005-05       Impact factor: 3.715

6.  In silico prediction and functional validation of sigma28-regulated genes in Chlamydia and Escherichia coli.

Authors:  Hilda Hiu Yin Yu; Dennis Kibler; Ming Tan
Journal:  J Bacteriol       Date:  2006-09-22       Impact factor: 3.490

7.  Mosaic structure of intragenic repetitive elements in histone H1-like protein Hc2 varies within serovars of Chlamydia trachomatis.

Authors:  Markus Klint; Mikael Thollesson; Erik Bongcam-Rudloff; Svend Birkelund; Anders Nilsson; Björn Herrmann
Journal:  BMC Microbiol       Date:  2010-03-17       Impact factor: 3.605

Review 8.  Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities.

Authors:  Anders Omsland; Barbara Susanne Sixt; Matthias Horn; Ted Hackstadt
Journal:  FEMS Microbiol Rev       Date:  2014-02-24       Impact factor: 16.408

9.  Translation inhibition of the developmental cycle protein HctA by the small RNA IhtA is conserved across Chlamydia.

Authors:  Jeremiah Tattersall; Geeta Vittal Rao; Justin Runac; Ted Hackstadt; Scott S Grieshaber; Nicole A Grieshaber
Journal:  PLoS One       Date:  2012-10-11       Impact factor: 3.240

10.  Identification of proteins associated with polyhydroxybutyrate granules from Herbaspirillum seropedicae SmR1--old partners, new players.

Authors:  Evandro F Tirapelle; Marcelo Müller-Santos; Michelle Z Tadra-Sfeir; Marco A S Kadowaki; Maria B R Steffens; Rose A Monteiro; Emanuel M Souza; Fabio O Pedrosa; Leda S Chubatsu
Journal:  PLoS One       Date:  2013-09-25       Impact factor: 3.240
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