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Literature DB >> 8727043

Mechanistics of formation and ultrastructural evaluation of hepatocyte spheroids.

M V Peshwa¹, F J Wu, H L Sharp, F B Cerra, W S Hu.

Abstract

Freshly harvested rat hepatocytes form spheroids on uncoated positively charged polystyrene surfaces. Time lapse microscopy revealed that cell movement and reorganization were involved in spheroid formation. Ultrastructural evaluation using scanning and transmission electron microscopy indicated polarized cellular morphology and extensive cell-cell communication within spheroids. Bile canalicular structures were observed to surround each individual hepatocyte, forming an intricate three-dimensional continuous network of channels that appeared to end as pores/holes on the surface of the spheroid. The maintenance of differentiated cellular morphology coincided with preservation of hepatocyte viability and enhanced levels of tissue specific functions in spheroids.

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Year: 1996 PMID： 8727043 DOI： 10.1007/BF02722946

Source DB: PubMed Journal: In Vitro Cell Dev Biol Anim ISSN： 1071-2690 Impact factor: 2.416

11 in total

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Journal: Biotechnol Bioeng Date: 1993-01-20 Impact factor: 4.530

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Journal: Exp Cell Res Date: 1990-02 Impact factor: 3.905

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Journal: Exp Cell Res Date: 1992-06 Impact factor: 3.905

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Authors: A Ben-Ze'ev; G S Robinson; N L Bucher; S R Farmer
Journal: Proc Natl Acad Sci U S A Date: 1988-04 Impact factor: 11.205

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Journal: Biochem Biophys Res Commun Date: 1989-05-30 Impact factor: 3.575

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Journal: Diabetes Date: 1987-07 Impact factor: 9.461

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Journal: Cell Transplant Date: 1993 Nov-Dec Impact factor: 4.064

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Journal: Biochim Biophys Acta Date: 1987-11-12

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Journal: Cell Struct Funct Date: 1988-04 Impact factor: 2.212

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21 in total

1. In vitro culture of rat hepatocytes without exogenous matrix.

Authors: M Barbich; A Lorenti; P Sorroche; E Mocetti; A Hidalgo; C B de Di Risio; S H Hyon; P Argibay
Journal: In Vitro Cell Dev Biol Anim Date: 2000 Jul-Aug Impact factor: 2.416

2. Three-Dimensional In Vitro Hepatic Constructs Formed Using Combinatorial Tapered Stencil for Cluster Culture (TASCL) Device.

Authors: Yoshitaka Miyamoto; Masashi Ikeuchi; Hirofumi Noguchi; Tohru Yagi; Shuji Hayashi
Journal: Cell Med Date: 2014-12-12

3. Spheroid Formation and Evaluation of Hepatic Cells in a Three-Dimensional Culture Device.

Authors: Yoshitaka Miyamoto; Masashi Ikeuchi; Hirofumi Noguchi; Tohru Yagi; Shuji Hayashi
Journal: Cell Med Date: 2015-08-26

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Authors: Maria Bokhari; Ross J Carnachan; Neil R Cameron; Stefan A Przyborski
Journal: J Anat Date: 2007-08-15 Impact factor: 2.610

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Authors: H Mizumoto; M Hayakami; K Nakazawa; H Ijima; K Funatsu
Journal: Cytotechnology Date: 1999-09 Impact factor: 2.058