| Literature DB >> 8724784 |
O A Stepanaia, E A Begunova, I M Tsfasman, I S Kulaev.
Abstract
The bacteriolytic peptidase L1 has been isolated from the enzyme preparation of lysoamidase capable to lyze cell walls of gram-positive bacteria using ion-exchange chromatography and gel filtration. Some physico-chemical properties of the enzyme have been established. The molecular mass of L1 is 21 kDa, the pH optimum for Staphylococcus aureus cell lysis is 7-11. The optimal concentration of the buffer is 50 mM; the temperature optimum is 70 degrees C; the half-inactivation temperature is 55 degrees C.Entities:
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Year: 1996 PMID： 8724784
Source DB: PubMed Journal: Biokhimiia ISSN： 0320-9725