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Literature DB >> 87240

Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test.

E F Hunter, R M McKinney, S E Maddison, D D Cruce.

Abstract

The fluorescent treponemal antibody absorption (FTA-ABS) double-staining procedure was reproducible, comparable to the conventional test, and easy to read. We recommend the use of the FTA-ABS double-staining procedure for microscopes with incident illumination, the 100 x/1.30 oil achromatic objective and the 6.3 x ocular to obtain optimal fluorescence, and the KP560 as a barrier filter to exclude rhodamine emission when fluorescein fluorescence is read. With this system, errors related to poor focusing or failure to visualise treponemes on all smears should be eliminated.

Entities: Chemical Disease

Mesh：

Substances：
Fluoresceins
Rhodamines

Year: 1979 PMID： 87240 PMCID： PMC1045600 DOI： 10.1136/sti.55.2.105

Source DB: PubMed Journal: Br J Vener Dis ISSN： 0007-134X

5 in total

1. The use of a vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incidental light.

Authors: J S Ploem
Journal: Z Wiss Mikrosk Date: 1967-11

2. Immunoglobulin specificity for the fluorescent treponemal antibody-absorption test conjugate.

Authors: E F Hunter; S E Maddison; S A Larsen; M B Felker; J C Feeley
Journal: J Clin Microbiol Date: 1976-10 Impact factor: 5.948

3. Comparative evaluation of commercial precipitating antisera against human IgM and IgM.

Authors: C B Reimer; D J Phillips; S E Maddison; S L Shore
Journal: J Lab Clin Med Date: 1970-12

4. Conjugation methods in immunofluorescence.

Authors: R McKinney; L Thacker; G A Hebert
Journal: J Dent Res Date: 1976-01 Impact factor: 6.116

5. Immunochemical parameters of some commercial conjugates for the fluorescent treponemal antibody-absorption test.

Authors: E F Hunter; J F Smith; J S Lewis; B E McGrew; J D Schmale
Journal: Infect Immun Date: 1972-06 Impact factor: 3.441

5 in total

9 in total

1. Novel point-of-care test for simultaneous detection of nontreponemal and treponemal antibodies in patients with syphilis.

Authors: Arnold R Castro; Javan Esfandiari; Shailendra Kumar; Matthew Ashton; Susan E Kikkert; Mahin M Park; Ronald C Ballard
Journal: J Clin Microbiol Date: 2010-09-29 Impact factor: 5.948

Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test.

1. The use of a vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incidental light.

2. Immunoglobulin specificity for the fluorescent treponemal antibody-absorption test conjugate.

3. Comparative evaluation of commercial precipitating antisera against human IgM and IgM.

4. Conjugation methods in immunofluorescence.

5. Immunochemical parameters of some commercial conjugates for the fluorescent treponemal antibody-absorption test.

1. Novel point-of-care test for simultaneous detection of nontreponemal and treponemal antibodies in patients with syphilis.

2. Percoll-purified Treponema pallidum, an improved fluorescent treponemal antibody-absorbed antigen.

3. Fluorescent treponemal antibody-absorption double-staining procedure.

4. Fluorescent treponemal antibody absorption double-staining test evaluation.

5. Further studies with the fluorescent treponemal antibody-absorption double-staining procedure.

Review 6. Laboratory diagnosis and interpretation of tests for syphilis.

7. Specific immunofluorescent staining of pathogenic treponemes with a monoclonal antibody.

8. Four-step enzyme-linked immunosorbent assay for detection of Treponema pallidum antibody.

9. Double-conjugate enzyme-linked immunosorbent assay for immunoglobulins G and M against Treponema pallidum.