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Literature DB >> 8723930

Single-tube protocol for the extraction of DNA or RNA from paraffin-embedded tissues using a starch-based adhesive.

G R Turbett¹, T C Barnett, E K Dillon, L N Sellner.

Abstract

We describe a method using an inexpensive craft glue to routinely isolate specific areas of tissue as small as 1 mm2 from paraffin sections. The tissue may be digested to release nucleic acid suitable for PCR or reverse transcription PCR. The use of this procedure obviates the requirement for manual microdissection or ultraviolet light irradiation. Tissue remaining on the slide can be stained and analyzed, allowing the precision of the extraction to be determined. The slide can be stored as a permanent record of the material taken for analysis.

Entities: Chemical

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Year: 1996 PMID： 8723930 DOI： 10.2144/96205st04

Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993

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Cited

6 in total

1. A new method for histological microdissection utilizing an ultrasonically oscillating needle: demonstrated by differential mRNA expression in human lung carcinoma tissue.

Authors: M Harsch; K Bendrat; G Hofmeier; D Branscheid; A Niendorf
Journal: Am J Pathol Date: 2001-06 Impact factor: 4.307

2. Analysis of T-cell clonality using laser capture microdissection and high-resolution microcapillary electrophoresis.

Authors: Evgeny Yakirevich; Cynthia L Jackson; Patricia A Meitner; Dolores MacKenzie; Rose Tavares; Leslie Robinson-Bostom; Ronald A DeLellis; Murray B Resnick
Journal: J Mol Diagn Date: 2007-07-09 Impact factor: 5.568

3. Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies.

Authors: Gretel Mendoza; Amelia Portillo; Jorge Olmos-Soto
Journal: Oncol Lett Date: 2012-10-22 Impact factor: 2.967

Single-tube protocol for the extraction of DNA or RNA from paraffin-embedded tissues using a starch-based adhesive.

1. A new method for histological microdissection utilizing an ultrasonically oscillating needle: demonstrated by differential mRNA expression in human lung carcinoma tissue.

2. Analysis of T-cell clonality using laser capture microdissection and high-resolution microcapillary electrophoresis.

3. Accurate breast cancer diagnosis through real-time PCR her-2 gene quantification using immunohistochemically-identified biopsies.

4. Molecular diagnosis of metastasizing oligodendroglioma: a case report.

5. Frameshift Mutations of HSPA4 and MED13 in Gastric and Colorectal Cancers.

6. Microsatellite instability and loss of heterozygosity in mammary carcinoma and its probable precursors.