Literature DB >> 872220

Cowpea mosaic virus RNAs have neither m7GpppN ... nor mono-, di- or triphosphates at their 5' ends.

J Klootwijk, I Klein, P Zabel, A van Kammen.   

Abstract

32P-labeled cowpea mosaic virus (CPMV) RNAs were analyzed for their 5' termini by three different fingerprinting standard procedures. These analyses showed unequivocally that CPMV RNAs lack a structure like m7GpppN ... at their 5' termini. In addition, neither mono-, di- or triphosphorylated [(p)(p)pN ....] termini were found in the respective fingerprints. The inability to label the 5' termini treatment with gamma-32P-ATP and polynucleotide kinase, either per se or after pretreatment with alkaline phosphatase, strongly suggests the absence of a free 5'-OH and a free 5'-phosphate at the 5' terminal nucleotide in CPMV RNAs. The various analyses also showed that no 2' -O-methylated nucleotides, N6-methyladenosines or pseudo-uridines are present in CPMV RNAs.

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Year:  1977        PMID： 872220     DOI： 10.1016/0092-8674(77)90318-x

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  22 in total

1.  A convenient sequencing method for 5' protein-linked RNAs.

Authors:  A Nomoto; N Imura
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971

2.  Determination of the length distribution of poly(A) at the 3' terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA.

Authors:  P Ahlquist; P Kaesberg
Journal:  Nucleic Acids Res       Date:  1979-11-10       Impact factor: 16.971

3.  Orientation of the cleavage map of the 200-kilodalton polypeptide encoded by the bottom-component RNA of cowpea mosaic virus.

Authors:  R Goldbach; G Rezelman
Journal:  J Virol       Date:  1983-05       Impact factor: 5.103

4.  Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts.

Authors:  G Rezelman; R Goldbach; A Van Kammen
Journal:  J Virol       Date:  1980-11       Impact factor: 5.103

5.  Analysis of terminal structures of RNA from potato virus X.

Authors:  N Sonenberg; A J Shatkin; R P Ricciardi; M Rubin; R M Goodman
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

6.  Association of a Mr 50,000 cap-binding protein with the cytoskeleton in baby hamster kidney cells.

Authors:  A Zumbé; C Stähli; H Trachsel
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

Review 7.  5'-terminal cap structure in eucaryotic messenger ribonucleic acids.

Authors:  A K Banerjee
Journal:  Microbiol Rev       Date:  1980-06

8.  In Vitro Replication of Cowpea Mosaic Virus RNA III. Template Recognition by Cowpea Mosaic Virus RNA Replicase.

Authors:  P Zabel; I Jongen-Neven; A van Kammen
Journal:  J Virol       Date:  1979-01       Impact factor: 5.103

9.  Determination of the proteolytic processing sites in the polyprotein encoded by the bottom-component RNA of cowpea mosaic virus.

Authors:  J Wellink; G Rezelman; R Goldbach; K Beyreuther
Journal:  J Virol       Date:  1986-07       Impact factor: 5.103

10.  A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.

Authors:  J Stanley; P Rottier; J W Davies; P Zabel; A Van Kammen
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971
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