AIM: To compare the haemoglobin (Hb) H inclusion test with a polymerase chain reaction (PCR) test in routine screening for alpha thalassaemia. METHODS: Ninety nine peripheral blood samples from Chinese patients with mean corpuscular volume below 80 fl were screened for alpha thalassaemia using the HbH inclusion test and by PCR utilising primers bridging the common deletion breakpoint of the South East Asian (--SEA/) deletion. RESULTS: The HbH inclusion test was positive in 78 (79%) patients, 73 (93.7%) of whom carried the (--SEA/) deletion on analysis of their DNA by PCR, as did one patient with a negative HbH inclusion test. CONCLUSIONS: These results suggest that in areas with a high prevalence of the (--SEA/) deletion, such as Hong Kong, the HbH inclusion test can be replaced by PCR as the investigation of choice in screening for alpha thalassaemia.
AIM: To compare the haemoglobin (Hb) H inclusion test with a polymerase chain reaction (PCR) test in routine screening for alpha thalassaemia. METHODS: Ninety nine peripheral blood samples from Chinese patients with mean corpuscular volume below 80 fl were screened for alpha thalassaemia using the HbH inclusion test and by PCR utilising primers bridging the common deletion breakpoint of the South East Asian (--SEA/) deletion. RESULTS: The HbH inclusion test was positive in 78 (79%) patients, 73 (93.7%) of whom carried the (--SEA/) deletion on analysis of their DNA by PCR, as did one patient with a negative HbH inclusion test. CONCLUSIONS: These results suggest that in areas with a high prevalence of the (--SEA/) deletion, such as Hong Kong, the HbH inclusion test can be replaced by PCR as the investigation of choice in screening for alpha thalassaemia.
Authors: K J Skogerboe; S F West; C Smith; S T Terashita; C N LeCrone; J C Detter; J F Tait Journal: Arch Pathol Lab Med Date: 1992-10 Impact factor: 5.534
Authors: W Tang; H Y Luo; M Albitar; M Patterson; B Eng; J S Waye; S A Liebhaber; D R Higgs; D H Chui Journal: Blood Date: 1992-07-15 Impact factor: 22.113
Authors: R Liang; S Liang; N H Jiang; X J Wen; J B Zhao; J F Nechtman; T A Stoming; T H Huisman Journal: Br J Haematol Date: 1994-02 Impact factor: 6.998
Authors: Shir Ying Lee; Crystal M E Chen; Elaine Y P Lim; Liang Shen; Aneesh Sathe; Aahan Singh; Jan Sauer; Kaveh Taghipour; Christina Y C Yip Journal: J Pathol Inform Date: 2021-04-07