BACKGROUND: It has been shown that MUC2 is the prominent mucin synthesised in healthy colon. AIM: To identify the predominant mucins in ulcerative colitis (UC) and to study their biosynthesis. METHODS AND RESULTS: Mucin was purified from UC resection specimens. This mucin on sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) presented as one, high molecular weight, periodic acid/Schiff's reagent (PAS) stainable band. Amino acid composition showed a close resemblance to that of MUC2. Immunoprecipitation with a specific anti-MUC2 antiserum confirmed that this mucin was MUC2. In addition, on the mRNA level MUC2 was also the most prominent mucin expressed in UC. Polyclonal antiserum was elicited, mainly recognising mucin peptide epitopes of UC and normal colonic mucin. Biosynthetic studies with [35S]amino acids showed that the MUC2-precursor in UC displayed a molecular mass on SDS-PAGE of approximately 600 kDa. This precursor was converted into a mature MUC2 with anomalous mobility on SDS-PAGE of 550 kDa and was secreted. Only this 550 kDa band could be labelled with [35S]sulphate and stained by PAS. CONCLUSIONS: This study shows that in parallel with the mucin expression in healthy controls, MUC2 is the major mucin expressed in UC. Qualitatively, MUC2 biosynthesis seems unchanged in UC.
BACKGROUND: It has been shown that MUC2 is the prominent mucin synthesised in healthy colon. AIM: To identify the predominant mucins in ulcerative colitis (UC) and to study their biosynthesis. METHODS AND RESULTS:Mucin was purified from UC resection specimens. This mucin on sodium dodecylsulphatepolyacrylamide gel electrophoresis (SDS-PAGE) presented as one, high molecular weight, periodic acid/Schiff's reagent (PAS) stainable band. Amino acid composition showed a close resemblance to that of MUC2. Immunoprecipitation with a specific anti-MUC2 antiserum confirmed that this mucin was MUC2. In addition, on the mRNA level MUC2 was also the most prominent mucin expressed in UC. Polyclonal antiserum was elicited, mainly recognising mucin peptide epitopes of UC and normal colonic mucin. Biosynthetic studies with [35S]amino acids showed that the MUC2-precursor in UC displayed a molecular mass on SDS-PAGE of approximately 600 kDa. This precursor was converted into a mature MUC2 with anomalous mobility on SDS-PAGE of 550 kDa and was secreted. Only this 550 kDa band could be labelled with [35S]sulphate and stained by PAS. CONCLUSIONS: This study shows that in parallel with the mucin expression in healthy controls, MUC2 is the major mucin expressed in UC. Qualitatively, MUC2 biosynthesis seems unchanged in UC.
Authors: N Porchet; V C Nguyen; J Dufosse; J P Audie; V Guyonnet-Duperat; M S Gross; C Denis; P Degand; A Bernheim; J P Aubert Journal: Biochem Biophys Res Commun Date: 1991-03-15 Impact factor: 3.575
Authors: J R Gum; J W Hicks; D M Swallow; R L Lagace; J C Byrd; D T Lamport; B Siddiki; Y S Kim Journal: Biochem Biophys Res Commun Date: 1990-08-31 Impact factor: 3.575
Authors: S J Gendler; C A Lancaster; J Taylor-Papadimitriou; T Duhig; N Peat; J Burchell; L Pemberton; E N Lalani; D Wilson Journal: J Biol Chem Date: 1990-09-05 Impact factor: 5.157
Authors: Mireille K Makkink; Nicole M J Schwerbrock; Michael Mähler; Jos A Boshuizen; Ingrid B Renes; Markus Cornberg; Hans J Hedrich; Alexandra W C Einerhand; Hans A Büller; Siegfried Wagner; Marie-Luise Enss; Jan Dekker Journal: Dig Dis Sci Date: 2002-10 Impact factor: 3.199
Authors: Christoph Moehle; Nikolaus Ackermann; Thomas Langmann; Charalampos Aslanidis; Alexander Kel; Olga Kel-Margoulis; Anna Schmitz-Madry; Alexandra Zahn; Wolfgang Stremmel; Gerd Schmitz Journal: J Mol Med (Berl) Date: 2006-10-21 Impact factor: 4.599
Authors: Kirk S B Bergstrom; Vanessa Kissoon-Singh; Deanna L Gibson; Caixia Ma; Marinieve Montero; Ho Pan Sham; Natasha Ryz; Tina Huang; Anna Velcich; B Brett Finlay; Kris Chadee; Bruce A Vallance Journal: PLoS Pathog Date: 2010-05-13 Impact factor: 6.823
Authors: M Faure; D Moënnoz; F Montigon; C Mettraux; S Mercier; E J Schiffrin; C Obled; D Breuillé; J Boza Journal: Dig Dis Sci Date: 2003-07 Impact factor: 3.199