OBJECTIVE: To determine the potential of 111In-labeled anti-E-selectin monoclonal antibody (MAb) to image localized endothelial activation in rheumatoid arthritis (RA). METHODS: Fourteen patients with RA were studied after intravenous administration of 111In-labeled F(ab')2 fragments of MAb against the cytokine-inducible endothelial cell activation antigen E-selectin (MAb 1.2B6). To compare uptake of 1.2B6 with that of nonspecific immunoglobulin, 111In-labeled polyclonal human immunoglobulin (HIG) was separately administered to 6 of these patients and the relative uptake of each tracer was determined. RESULTS: Prominent and discrete uptake of the radiolabeled MAb 1.2B6 was clearly visible in inflamed joints of all patients. Compared with 111In-HIG, 111In-1.2B6 provided superior images in terms of sensitivity and image intensity. Furthermore, the distribution of uptake in inflamed joints was different for the 2 tracers, with 1.2B6 showing a more focal localization in synovium. CONCLUSION: This study demonstrates that it is possible to objectively assess E-selectin expression on activated endothelium in vivo in patients with RA, using a radiolabeled MAb. This technique has considerable potential for monitoring disease activity and response to therapy in inflammatory diseases.
OBJECTIVE: To determine the potential of 111In-labeled anti-E-selectin monoclonal antibody (MAb) to image localized endothelial activation in rheumatoid arthritis (RA). METHODS: Fourteen patients with RA were studied after intravenous administration of 111In-labeled F(ab')2 fragments of MAb against the cytokine-inducible endothelial cell activation antigen E-selectin (MAb 1.2B6). To compare uptake of 1.2B6 with that of nonspecific immunoglobulin, 111In-labeled polyclonal human immunoglobulin (HIG) was separately administered to 6 of these patients and the relative uptake of each tracer was determined. RESULTS: Prominent and discrete uptake of the radiolabeled MAb 1.2B6 was clearly visible in inflamed joints of all patients. Compared with 111In-HIG, 111In-1.2B6 provided superior images in terms of sensitivity and image intensity. Furthermore, the distribution of uptake in inflamed joints was different for the 2 tracers, with 1.2B6 showing a more focal localization in synovium. CONCLUSION: This study demonstrates that it is possible to objectively assess E-selectin expression on activated endothelium in vivo in patients with RA, using a radiolabeled MAb. This technique has considerable potential for monitoring disease activity and response to therapy in inflammatory diseases.
Authors: Harshad S Sakhalkar; Milind K Dalal; Aliasger K Salem; Ramin Ansari; Jie Fu; Mohammad F Kiani; David T Kurjiaka; Justin Hanes; Kevin M Shakesheff; Douglas J Goetz Journal: Proc Natl Acad Sci U S A Date: 2003-12-10 Impact factor: 11.205
Authors: Paulo Henrique Rosado-de-Castro; Sergio Augusto Lopes de Souza; Dângelo Alexandre; Lea Mirian Barbosa da Fonseca; Bianca Gutfilen Journal: World J Orthop Date: 2014-07-18
Authors: Aman P Mann; Anoma Somasunderam; René Nieves-Alicea; Xin Li; Austin Hu; Anil K Sood; Mauro Ferrari; David G Gorenstein; Takemi Tanaka Journal: PLoS One Date: 2010-09-30 Impact factor: 3.240
Authors: F Conti; G Malviya; F Ceccarelli; R Priori; A Iagnocco; G Valesini; A Signore Journal: Eur J Nucl Med Mol Imaging Date: 2012-05-16 Impact factor: 9.236
Authors: G Malviya; F Conti; M Chianelli; F Scopinaro; R A Dierckx; A Signore Journal: Eur J Nucl Med Mol Imaging Date: 2009-09-24 Impact factor: 9.236