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Literature DB >> 869934

Protein chromatography on adsorbents with hydrophobic and ionic groups. Purification of human erythrocyte glycophorin.

Abstract

Human erythrocyte glycophorin was purified rapidly by (a) chromatography of a Triton X-100 extract of erythrocyte 'ghosts' on N-(3-carboxypropionyl)aminodecyl-Sepharose in buffers containing Triton X-100 or sodium dodecyl sulphate, or (b) chromatography of whole 'ghosts', solubilized in sodium dodecyl sulphate, on dodecyl-Sepharose, in buffers containing sodium dodecyl sulphate. The products contained 85-95% glycophorin (electrophoretic band PAS-1) and the major contaminants were glycoproteins PAS-2 (possibly a subunit of glycophorin) and PAS-3.

Entities: Chemical Gene Species

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Year: 1977 PMID： 869934 PMCID： PMC1164711 DOI： 10.1042/bj1630397

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

16 in total

4. Protein chromatography on adsorbents with hydrophobic and ionic groups. Some properties of N-(3-carboxypropionyl)aminodecyl-sepharose and its interaction with wheat-germ aspartate transcarbamoylase.

Authors: R J Yon; R J Simmonds
Journal: Biochem J Date: 1975-11 Impact factor: 3.857

5. Protein chromatography on adsorbents with hydrophobic and ionic groups. Chromatography of dodecyl sulphate-solubilized proteins of the human erythrocyte membrane on N-(3-carboxypropionyl)aminodecyl-sepharose.

Authors: R J Simmonds; R J Yon
Journal: Biochem J Date: 1976-07-01 Impact factor: 3.857

6. Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis. A method to validate molecular weight estimates.

Authors: G A Banker; C W Cotman
Journal: J Biol Chem Date: 1972-09-25 Impact factor: 5.157

Protein chromatography on adsorbents with hydrophobic and ionic groups. Purification of human erythrocyte glycophorin.

1. The major sialoglycoprotein of the human erythrocyte membrane. Release with a non-ionic detergent and purification.

2. Protein measurement with the Folin phenol reagent.

3. Heterogeneity of the sialoglycoproteins of the normal human erythrocyte membrane.

4. Protein chromatography on adsorbents with hydrophobic and ionic groups. Some properties of N-(3-carboxypropionyl)aminodecyl-sepharose and its interaction with wheat-germ aspartate transcarbamoylase.

5. Protein chromatography on adsorbents with hydrophobic and ionic groups. Chromatography of dodecyl sulphate-solubilized proteins of the human erythrocyte membrane on N-(3-carboxypropionyl)aminodecyl-sepharose.

6. Measurement of free electrophoretic mobility and retardation coefficient of protein-sodium dodecyl sulfate complexes by gel electrophoresis. A method to validate molecular weight estimates.

7. Glycoprotein staining following electrophoresis on acrylamide gels.

8. Major human erythrocyte glycoprotein spans the cell membrane.

9. Subunit structure of human erythrocyte glycophorin A.

Review 10. The organization of proteins in the human red blood cell membrane. A review.

1. The adsorption of proteins and protein--dodecyl sulphate complexes on N-(3-carboxypropionyl)aminodecyl-Sepharose.