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Literature DB >> 869931

A simple method for purification of epoxide hydratase from rat liver.

Abstract

Rat liver epoxide hydratase was purified 460-fold to homogeneity by detergent solubilization and ion-exchange chromatography. The enzyme obtained in high yield (36%) exhibited a specific activity of 479nmol of styrene glycol formed/min per mg of protein, with styrene oxide as substrate. Only one polypeptide-staining band, mol.wt. 49500, was visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.

Entities: Chemical Gene Species

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Year: 1977 PMID： 869931 PMCID： PMC1164707 DOI： 10.1042/bj1630381

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

10 in total

1. Purification of rat liver epoxide hydratase to apparent homogeneity.

Authors: P Bentley; F Oesch
Journal: FEBS Lett Date: 1975-11-15 Impact factor: 4.124

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. Liver microsomal expoxide hydrase. Solubilization, purification, and characterization.

Authors: A Y Lu; D Ryan; D M Jerina; J W Daly; W Levin
Journal: J Biol Chem Date: 1975-10-25 Impact factor: 5.157

Review 4. Mammalian epoxide hydrases: inducible enzymes catalysing the inactivation of carcinogenic and cytotoxic metabolites derived from aromatic and olefinic compounds.

Authors: F Oesch
Journal: Xenobiotica Date: 1973-05 Impact factor: 1.908

5. Metabolic activation of benzo(a)pyrene proceeds by a diol-epoxide.

Authors: P Sims; P L Grover; A Swaisland; K Pal; A Hewer
Journal: Nature Date: 1974-11-22 Impact factor: 49.962

6. Arene oxides: a new aspect of drug metabolism.

Authors: D M Jerina; J W Daly
Journal: Science Date: 1974-08-16 Impact factor: 47.728

7. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

8. Studies on the purification of rat liver uridine diphosphate glucuronyltransferase.

Authors: B Burchell
Journal: Biochem J Date: 1977-03-01 Impact factor: 3.857

9. Latency of epoxide hydratase and its relationship to that of UDPglucuronyltransferase.

Authors: B Burchell; P Bentley; F Oesch
Journal: Biochim Biophys Acta Date: 1976-09-24

10. Purification and specificity of a human microsomal epoxide hydratase.

Authors: F Oesch
Journal: Biochem J Date: 1974-04 Impact factor: 3.857

10 in total

8 in total

1. Multiplicity of induction patterns of rat liver microsomal mono-oxygenases and other polypeptides produced by administration of various xenobiotics.

Authors: R N Sharma; R G Cameron; E Farber; M J Griffin; J G Joly; R K Murray
Journal: Biochem J Date: 1979-08-15 Impact factor: 3.857

2. Microsomal epoxide hydrolase of rat liver. Purification and characterization of enzyme fractions with different chromatographic characteristics.

Authors: N J Bulleid; A B Graham; J A Craft
Journal: Biochem J Date: 1986-01-15 Impact factor: 3.857

3. Stabilization of glucose-6-phosphatase activity by a 21 000-dalton hepatic microsomal protein.

Authors: A Burchell; B Burchell; M Monaco; H E Walls; W J Arion
Journal: Biochem J Date: 1985-09-01 Impact factor: 3.857

4. Substrate specificity and properties of uridine diphosphate glucuronyltransferase purified to apparent homogeneity from phenobarbital-treated rat liver.

Authors: B Burchell
Journal: Biochem J Date: 1978-09-01 Impact factor: 3.857