Literature DB >> 8698104

Apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pig ovaries.

N Manabe1, Y Imai, H Ohno, Y Takahagi, M Sugimoto, H Miyamoto.   

Abstract

The porcine antral follicles, 3-6 mm in diameter, were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease and DNA ladder formation, estimated by electrophoresis, were noted in granulosa cells but not in cumulus cells. Extremely low activity of the endonuclease and no DNA ladder formation were observed in both types of cells obtained from healthy follicles. Moreover, apoptotic cells were observed histochemically among granulosa cells only. A good correlation (r = 0.987) between the endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle was found. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs.

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Year:  1996        PMID： 8698104     DOI： 10.1007/bf01925566

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  15 in total

1.  Loss of follicles during the follicular phase of the estrous cycle of swine as affected by genetic group and level of feed intake.

Authors:  R A Dailey; J R Clark; N L First; A B Chapman; L E Casida
Journal:  J Anim Sci       Date:  1975-09       Impact factor: 3.159

2.  Apoptosis. The role of the endonuclease.

Authors:  M J Arends; R G Morris; A H Wyllie
Journal:  Am J Pathol       Date:  1990-03       Impact factor: 4.307

3.  Simple and rapid fluorimetric method for DNA microassay.

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Journal:  Anal Biochem       Date:  1977-11       Impact factor: 3.365

4.  Developmental expression of Ca++/Mg++-dependent endonuclease activity in rat granulosa and luteal cells.

Authors:  A J Zeleznik; L L Ihrig; S G Bassett
Journal:  Endocrinology       Date:  1989-10       Impact factor: 4.736

5.  Microscale autoradiographic method for the qualitative and quantitative analysis of apoptotic DNA fragmentation.

Authors:  J L Tilly; A J Hsueh
Journal:  J Cell Physiol       Date:  1993-03       Impact factor: 6.384

6.  Flow cytometric determination of degraded deoxyribonucleic acid in granulosa cells to identify atretic follicles during preovulatory maturation in the pig.

Authors:  H D Guthrie; G R Welch; B S Cooper; A D Zakaria; L A Johnson
Journal:  Biol Reprod       Date:  1994-06       Impact factor: 4.285

7.  Steroidogenesis in porcine atretic follicles: loss of aromatase activity in isolated granulosa and theca.

Authors:  W S Maxson; A F Haney; D W Schomberg
Journal:  Biol Reprod       Date:  1985-09       Impact factor: 4.285

8.  Interaction of estrogen and gonadotrophins on follicular atresia.

Authors:  S M Harman; J P Louvet; G T Ross
Journal:  Endocrinology       Date:  1975-05       Impact factor: 4.736

9.  Correlation of follicular steroid hormone profiles with ovarian cyclicity in sows.

Authors:  G O Babalola; B H Shapiro
Journal:  J Reprod Fertil       Date:  1988-09

10.  Atresia in follicles grown after ovulation in the pig: measurement of increased apoptosis in granulosa cells and reduced follicular fluid estradiol-17 beta.

Authors:  H D Guthrie; B S Cooper; G R Welch; A D Zakaria; L A Johnson
Journal:  Biol Reprod       Date:  1995-04       Impact factor: 4.285
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  7 in total

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Journal:  PLoS One       Date:  2012-06-21       Impact factor: 3.240

4.  Expression and preliminary functional profiling of the let-7 family during porcine ovary follicle atresia.

Authors:  Rui Cao; Wang Jun Wu; Xiao Long Zhou; Peng Xiao; Yi Wang; Hong Lin Liu
Journal:  Mol Cells       Date:  2015-03-31       Impact factor: 5.034

5.  The effect of lysophosphatidic acid during in vitro maturation of bovine oocytes: embryonic development and mRNA abundances of genes involved in apoptosis and oocyte competence.

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Journal:  Mediators Inflamm       Date:  2014-03-04       Impact factor: 4.711

6.  Prohibitin (PHB) inhibits apoptosis in rat granulosa cells (GCs) through the extracellular signal-regulated kinase 1/2 (ERK1/2) and the Bcl family of proteins.

Authors:  Indrajit Chowdhury; Winston E Thompson; Crystal Welch; Kelwyn Thomas; Roland Matthews
Journal:  Apoptosis       Date:  2013-12       Impact factor: 4.677

7.  Autophagy and Apoptosis of Porcine Ovarian Granulosa Cells During Follicular Development.

Authors:  Yuxin Zheng; Lizhu Ma; Ning Liu; Xiaorong Tang; Shun Guo; Bin Zhang; Zhongliang Jiang
Journal:  Animals (Basel)       Date:  2019-12-10       Impact factor: 2.752
  7 in total

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