OBJECTIVE: To clarify the relationship between the HLA-DR genotype and susceptibility to rheumatoid arthritis (RA) in Japanese patients. METHODS: HLA-DR typing and DRB1* genotyping were carried out by PCR and PCR-SSCP (single stranded DNA conformation polymorphism), respectively. RESULTS: In RA, the prevalence of HLA-DR4 was significantly higher (57.3%, p < 0.05). In particular, DRB1*0405 was predominantly higher (46.9%, p < 0.05) and DRB1*0401 was also increased although not significantly. HLA-DR8, especially DRB1*0802, was significantly lower (1.0%, p < 0.01). RA patients homozygous for DRB1*0405 showed slightly higher values for the erythrocyte sedimentation rate, gamma-globulin, and IgG, as well as positivity for rheumatoid factor and high titers for the Waalar-Rose test, and a decrease in the albumin/globulin ratio, albumin, and hemoglobin in comparison to patients without RA susceptibility genes, although the difference for each of these parameters was not significant. CONCLUSION: DRB1*0405 and DRB1*0802, which are both rare alleles in Caucasians, are positively and negatively correlated, respectively, with the pathogenesis of RA in Japan.
OBJECTIVE: To clarify the relationship between the HLA-DR genotype and susceptibility to rheumatoid arthritis (RA) in Japanese patients. METHODS: HLA-DR typing and DRB1* genotyping were carried out by PCR and PCR-SSCP (single stranded DNA conformation polymorphism), respectively. RESULTS: In RA, the prevalence of HLA-DR4 was significantly higher (57.3%, p < 0.05). In particular, DRB1*0405 was predominantly higher (46.9%, p < 0.05) and DRB1*0401 was also increased although not significantly. HLA-DR8, especially DRB1*0802, was significantly lower (1.0%, p < 0.01). RApatients homozygous for DRB1*0405 showed slightly higher values for the erythrocyte sedimentation rate, gamma-globulin, and IgG, as well as positivity for rheumatoid factor and high titers for the Waalar-Rose test, and a decrease in the albumin/globulin ratio, albumin, and hemoglobin in comparison to patients without RA susceptibility genes, although the difference for each of these parameters was not significant. CONCLUSION:DRB1*0405 and DRB1*0802, which are both rare alleles in Caucasians, are positively and negatively correlated, respectively, with the pathogenesis of RA in Japan.
Authors: Luis Francisco Valdés-Corona; Susana Hernández-Doño; Tatiana Sofia Rodríguez-Reyna; Rafael García-Silva; Juan Jakez; Monica Escamilla-Tilch; Guadalupe Lima; Luis Llorente; Carlos Pineda; Edmond Yunis; Julio Granados Journal: J Transl Autoimmun Date: 2020-05-15
Authors: T Rashid; M Leirisalo-Repo; Y Tani; S Hukuda; S Kobayashi; C Wilson; S Bansal; A Ebringer Journal: Clin Rheumatol Date: 2004-02-13 Impact factor: 2.980
Authors: Jade E Hollis-Moffatt; Kerry A Rowley; Amanda J Phipps-Green; Marilyn E Merriman; Nicola Dalbeth; Peter Gow; Andrew A Harrison; John Highton; Peter B B Jones; Lisa K Stamp; Pille Harrison; B Paul Wordsworth; Tony R Merriman Journal: Arthritis Res Ther Date: 2009-10-09 Impact factor: 5.156