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Literature DB >> 8694780

Temperature-dependence of open-complex formation at two Escherichia coli promoters with extended -10 sequences.

H D Burns¹, T A Belyaeva, S J Busby, S D Minchin.

Abstract

We have studied the formation of open complexes between purified RNA polymerase from Escherichia coli and DNA fragments carrying the galP1 promoter, a promoter with an extended -10 region. Unusually, these complexes are formed readily at low temperatures. This low-temperature opening is unaffected by deletions of either upstream or downstream promoter sequences. We conclude that low-temperature open-complex formation is due to specific base sequences in and just upstream of the extended -10 region. In contrast, open complexes are not formed at low temperatures with DNA fragments carrying the E. coli cysG promoter, which also has an extended -10 region. This demonstrates that an extended -10 sequence alone is not sufficient for low-temperature opening. Additionally, we report the temperature dependence of a hybrid galP1-cysG promoter, the related galP2 and galP3 promoters and a derivative of galP1 with an improved -10 hexamer sequence.

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Year: 1996 PMID： 8694780 PMCID： PMC1217479 DOI： 10.1042/bj3170305

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

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