BACKGROUND: The purpose of this study was to investigate the effect of complete intraluminal placement on prosthetic graft healing. METHODS: Thirty dogs underwent infrarenal abdominal aorta polytetrafluoroethylene interposition (12) or intraluminal stented (18) grafting. Grafts were removed at 4 and 8 weeks. Length of endothelial ingrowth and intima to media height ratios (IMHRs) were calculated. Perianastomotic endothelial (CD31+, factor VIII [FVIII+]), smooth muscle (actin+), macrophage (CD44+), and proliferating (PCNA+) cell content was determined. RESULTS: In control grafts mean proximal and distal anastomotic endothelial cell ingrowth was 0.42 +/- 0.06 and 0.47 +/- 0.08 cm at 4 weeks and 1.10 +/- 0.24 and 0.94 +/- 0.17 cm at 8 weeks. In intraluminal grafts mean proximal and distal anastomotic endothelial cell ingrowth was 1.57 +/- 0.09 and 1.54 +/- 0.12 cm at 4 weeks and 1.88 +/- 0.06 and 2.11 +/- 0.25 cm at 8 weeks. Endothelial ingrowth was greater in all stented grafts (p < 0.001). Mean proximal anastomosis IMHRs were 1.01 +/- 0.16 for 4-week and 1.42 +/- 0.16 for 8-week control grafts and 0.59 +/- 0.18 for 4-week and 0.50 +/- 0.14 for 8-week stented grafts. Similar IMHR values were present at the distal anastomosis. Lower IMHRs were observed in stented grafts (p < 0.05). Content of CD44+, PCNA+, and FVIII+ cells were reduced both proximally and distally in 4-week stented grafts (p < 0.05). Distal content of CD31+ and actin+ cells was greater in 4-week stented grafts (p < 0.05). At 8 weeks CD44+ cell content decreased in controls (p < 0.05). CONCLUSIONS: Intraluminal location enhances prosthetic graft reendothelialization and attenuates intimal thickening.
BACKGROUND: The purpose of this study was to investigate the effect of complete intraluminal placement on prosthetic graft healing. METHODS: Thirty dogs underwent infrarenal abdominal aorta polytetrafluoroethylene interposition (12) or intraluminal stented (18) grafting. Grafts were removed at 4 and 8 weeks. Length of endothelial ingrowth and intima to media height ratios (IMHRs) were calculated. Perianastomotic endothelial (CD31+, factor VIII [FVIII+]), smooth muscle (actin+), macrophage (CD44+), and proliferating (PCNA+) cell content was determined. RESULTS: In control grafts mean proximal and distal anastomotic endothelial cell ingrowth was 0.42 +/- 0.06 and 0.47 +/- 0.08 cm at 4 weeks and 1.10 +/- 0.24 and 0.94 +/- 0.17 cm at 8 weeks. In intraluminal grafts mean proximal and distal anastomotic endothelial cell ingrowth was 1.57 +/- 0.09 and 1.54 +/- 0.12 cm at 4 weeks and 1.88 +/- 0.06 and 2.11 +/- 0.25 cm at 8 weeks. Endothelial ingrowth was greater in all stented grafts (p < 0.001). Mean proximal anastomosis IMHRs were 1.01 +/- 0.16 for 4-week and 1.42 +/- 0.16 for 8-week control grafts and 0.59 +/- 0.18 for 4-week and 0.50 +/- 0.14 for 8-week stented grafts. Similar IMHR values were present at the distal anastomosis. Lower IMHRs were observed in stented grafts (p < 0.05). Content of CD44+, PCNA+, and FVIII+ cells were reduced both proximally and distally in 4-week stented grafts (p < 0.05). Distal content of CD31+ and actin+ cells was greater in 4-week stented grafts (p < 0.05). At 8 weeks CD44+ cell content decreased in controls (p < 0.05). CONCLUSIONS: Intraluminal location enhances prosthetic graft reendothelialization and attenuates intimal thickening.
Authors: Hyun Beom Kim; Young Ho Choi; Young Ho So; Seung-Kee Min; Hyo-Cheol Kim; Young Il Kim; Jae Hyung Park; Jin Wook Chung Journal: J Korean Med Sci Date: 2012-10-02 Impact factor: 2.153
Authors: Young Il Kim; Young Ho Choi; Jin Wook Chung; Hyo-Cheol Kim; Young Ho So; Hyun Beom Kim; Seung-Kee Min; Jae Hyung Park Journal: Korean J Radiol Date: 2014-09-12 Impact factor: 3.500
Authors: Randall J Smith; Bita Nasiri; Julien Kann; Donald Yergeau; Jonathan E Bard; Daniel D Swartz; Stelios T Andreadis Journal: Nat Commun Date: 2020-04-01 Impact factor: 14.919